Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gaikwald, S.M.; Keskar, S.S.; Khan, M.I.
    Purification and characterization of alpha-D-mannosidase from Aspergillus sp. (1995), Biochim. Biophys. Acta, 1250, 144-148.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 alpha-D-mannopyranose 55% inhibition at 30 mM Aspergillus sp.
3.2.1.24 Co2+ 46% inhibition at 1 mM Aspergillus sp.
3.2.1.24 Cu2+ complete inhibition at 1 mM Aspergillus sp.
3.2.1.24 D-glucose 67% inhibition at 30 mM Aspergillus sp.
3.2.1.24 Fe2+ 54% inhibition at 1 mM Aspergillus sp.
3.2.1.24 Hg2+ complete inhibition at 1 mM Aspergillus sp.
3.2.1.24 Mn2+ slight inhibition at 1 mM Aspergillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.083
-
p-nitrophenyl-alpha-D-mannopyranoside
-
Aspergillus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 extracellular
-
Aspergillus sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 69200
-
6 * 69200, SDS-PAGE Aspergillus sp.
3.2.1.24 412000
-
gel filtration Aspergillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 alpha-(1-2)-mannobiose + H2O Aspergillus sp.
-
alpha-D-mannopyranose + D-mannopyranose
-
?
3.2.1.24 alpha-(1-3)-mannobiose + H2O Aspergillus sp. highest activity and affinity alpha-D-mannopyranose + D-mannopyranose
-
?
3.2.1.24 alpha-(1-6)-mannobiose Aspergillus sp. low activity alpha-D-mannopyranose + D-mannopyranose
-
?
3.2.1.24 Man6-glycopeptide + H2O Aspergillus sp.
-
alpha-D-mannopyranose + ?
-
?
3.2.1.24 Man9-glycopeptide + H2O Aspergillus sp.
-
alpha-D-mannopyranose + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Aspergillus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.24 glycoprotein 3.8% neutral sugar content Aspergillus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, preparative PAGE Aspergillus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 cell culture
-
Aspergillus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 0.36
-
-
Aspergillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 alpha-(1-2)-mannobiose + H2O
-
Aspergillus sp. alpha-D-mannopyranose + D-mannopyranose
-
?
3.2.1.24 alpha-(1-3)-mannobiose + H2O highest activity and affinity Aspergillus sp. alpha-D-mannopyranose + D-mannopyranose
-
?
3.2.1.24 alpha-(1-6)-mannobiose low activity Aspergillus sp. alpha-D-mannopyranose + D-mannopyranose
-
?
3.2.1.24 alpha-(1-6)-mannobiose + H2O low activity Aspergillus sp. alpha-D-mannopyranose
-
?
3.2.1.24 Man6-glycopeptide + H2O
-
Aspergillus sp. alpha-D-mannopyranose + ?
-
?
3.2.1.24 Man9-glycopeptide + H2O
-
Aspergillus sp. alpha-D-mannopyranose + ?
-
?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Aspergillus sp. p-nitrophenol + alpha-D-mannopyranose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 hexamer 6 * 69200, SDS-PAGE Aspergillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.24 50
-
-
Aspergillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 25
-
stable for 16 h Aspergillus sp.
3.2.1.24 50
-
stable for 30 min at pH 6.0 Aspergillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6 6.5
-
Aspergillus sp.