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Literature summary extracted from
- Isolation and characterization of a new enzyme choline sulfatase (1961), J. Biochem., 50, 245-255.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.6 | Choline sulfate | - |
Pseudomonas nitroreducens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.6 | choline chloride | some inhibition at higher concentration | Pseudomonas nitroreducens | |
| 3.1.6.6 | F- | partial inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | sulfite | - |
Pseudomonas nitroreducens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.6 | 40 | - |
Choline sulfate | - |
Pseudomonas nitroreducens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.6 | Ca2+ | slight inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | Co2+ | complete inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | Cu2+ | complete inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | Hg2+ | complete inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | Mg2+ | slight inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | Mn2+ | slight inhibition | Pseudomonas nitroreducens | |
| 3.1.6.6 | Zn2+ | complete inhibition | Pseudomonas nitroreducens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.6 | choline sulfate + H2O | Pseudomonas nitroreducens | substrate-induced | choline + sulfate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.6 | Pseudomonas nitroreducens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.6 | partial | Pseudomonas nitroreducens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 0.0714 | - |
- |
Pseudomonas nitroreducens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.6 | amylose polysulfate + H2O | slightly hydrolyzed | Pseudomonas nitroreducens | amylose + sulfate | - |
? | |
| 3.1.6.6 | choline sulfate + H2O | - |
Pseudomonas nitroreducens | choline + sulfate | - |
? | |
| 3.1.6.6 | choline sulfate + H2O | substrate-induced | Pseudomonas nitroreducens | choline + sulfate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 37 | - |
assay | Pseudomonas nitroreducens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 8.3 | - |
- |
Pseudomonas nitroreducens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 6 | 10 | most stable at pH 6.6 | Pseudomonas nitroreducens |
| 3.1.6.6 | 6 | 10 | inactvation af the enzyme at lower pH's, pH 10: about 50% of activity maximum | Pseudomonas nitroreducens |
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