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Literature summary extracted from

  • Agogbua, S.I.O.; Wynn, C.H.
    Purification and properties of arylsulphatase B of human liver (1976), Biochem. J., 153, 415-421.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.6.12 dithiothreitol
-
 Homo sapiens
3.1.6.12 EDTA 60% activation at 10 mM Homo sapiens

General Stability

EC Number General Stability Organism
3.1.6.12 freezing, purified enzyme, rapid loss of activity, after 24 h irreversible Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.12 Ca2+
-
 Homo sapiens
3.1.6.12 Cu2+ bivalent cations are inhibitory Homo sapiens
3.1.6.12 iodoacetate
-
 Homo sapiens
3.1.6.12 Mg2+
-
 Homo sapiens
3.1.6.12 Mn2+ slight Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.12 0.8
-
 nitrocatechol sulfate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.12 47000 54000 gel filtration Homo sapiens
3.1.6.12 57000 61000 sedimentation equilibrium Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O Homo sapiens acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.12 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.1.6.12 photooxidation, with 1 mM Rose Bengal, 80% inactivation, no inactivation in presence of 1 mM pyridoxal phosphate and 30 mM 4-nitrocatechol Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.12
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.12 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Homo sapiens N-acetyl-D-galactosamine units + sulfate
-
 ?
3.1.6.12 p-nitrocatechol sulfate + H2O enzymes catalyze in addition to reaction of EC 3.1.6.12 also arylsulfatase reaction , see also EC 3.1.6.1 Homo sapiens p-nitrocatechol + sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.12 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.12 6.1
-
 nitrocatechol sulfate Homo sapiens