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Literature summary extracted from

  • Amoscato, A.A.; Brumfield, A.M.; Sansoni, S.B.; Herberman, R.B.; Chambers, W.H.
    Natural killer cell cytolytic granule-associated enzymes. I. Purification, characterization, and analyis of function of an enzyme with sulfatase activity (1991), J. Immunol., 147, 950-958.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 PO43-
-
 Rattus norvegicus
3.1.6.1 SDS
-
 Rattus norvegicus
3.1.6.1 SO32-
-
 Rattus norvegicus
3.1.6.1 SO42-
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.1 1.1
-
 4-nitrocatechol sulfate
-
 Rattus norvegicus
3.1.6.1 26
-
 4-nitrophenyl sulfate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.1 specific granule
-
 Rattus norvegicus 42581
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.1 40000
-
 the enzyme has the ability to complex to molecular masses of more than 300000, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.1 leukemia cell line
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.1 0.23
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 4-nitrocatechol sulfate + H2O
-
 Rattus norvegicus 4-nitrocatechol + sulfate
-
 ?
3.1.6.1 4-nitrophenyl sulfate + H2O
-
 Rattus norvegicus 4-nitrophenol + sulfate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.6.1 37
-
 5 h, 30% loss of activity Rattus norvegicus
3.1.6.1 56
-
 5 h, 51% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.1 5.5 6 4-nitrophenyl sulfate Rattus norvegicus
3.1.6.1 5.6
-
 hydrolysis of 3-nitrocatechol sulfate Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.6.1 5 7 pH 5.0: about 55% of maximal activity, pH 7.0: about 35% of maximal activity, hydrolysis of 4-nitrophenyl sulfate Rattus norvegicus
3.1.6.1 5 7 pH 5.0: about 75% of maximal activity, pH 7.0: about 45% of maximal activity, hydrolysis of 4-nitrocatechol sulfate Rattus norvegicus