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Literature summary extracted from

  • Quirk, S.; Do, B.T.
    Cloning, purification, and characterization of the Shigella boydii dGTP triphosphohydrolase (1997), J. Biol. Chem., 272, 332-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.5.1
-
 Shigella boydii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.5.1 initial conditions, vapor diffusion method Shigella boydii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.5.1 2-mercaptoethanol prior incubation, loss of thermostability Shigella boydii
3.1.5.1 dithiothreitol prior incubation, 12% of activity remains, loss of thermostability Shigella boydii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.5.1 59400
-
 homotetramer, 4 * 59400 Shigella boydii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.5.1 dGTP + H2O Shigella boydii
-
 deoxyguanosine + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.5.1 Shigella boydii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.5.1 recombinant protein to homogeneity Shigella boydii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.5.1 dGTP + H2O
-
 Shigella boydii deoxyguanosine + triphosphate
-
 ?
3.1.5.1 dGTP + H2O highly specific for Shigella boydii deoxyguanosine + triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.5.1 tetramer homotetramer, 4 * 59400 Shigella boydii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.5.1 60
-
 loss of thermostability in presence of reducing agents, 5% of activity remains Shigella boydii