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Literature summary extracted from
- A unique deoxyguanosine triphosphatase is responsible for the optA1 phenotype of Escherichia coli (1988), Proc. Natl. Acad. Sci. USA, 85, 2563-2567.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.5.1 | more stable at higher protein concentrations | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.5.1 | 0.002 | 0.005 | dGTP | - |
Escherichia coli |  |
| 3.1.5.1 | 0.15 | - |
GTP | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.5.1 | dGTP + H2O | Escherichia coli | - |
deoxyguanosine + triphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.5.1 | Escherichia coli | - |
mutant optA1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.5.1 | 16000 fold to homogeneity | Escherichia coli |
| 3.1.5.1 | high affinity for DNA | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.5.1 | 0 to -20°C, 0.1 mg/ml protein concentration, 30 days, 20% and 60% loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.5.1 | dGTP + H2O | - |
Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
| 3.1.5.1 | dGTP + H2O | highly specific for | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
| 3.1.5.1 | dGTP + H2O | no activity on dGDP | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
| 3.1.5.1 | GTP + H2O | - |
Escherichia coli | guanosine + triphosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.5.1 | More | 50 fold increased level | Escherichia coli |
| 3.1.5.1 | More | C-T transition 74 bases upstream | Escherichia coli |
| 3.1.5.1 | More | OPTA1 mutants | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.5.1 | - |
- |
reversible loss of activity at low protein concentrations | Escherichia coli |
| 3.1.5.1 | 55 | 60 | full activity after 1 h | Escherichia coli |
| 3.1.5.1 | 65 | 70 | 90% denatured after 1 h | Escherichia coli |
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Release 2023.1 (January 2023)
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