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Literature summary extracted from
- The purification and properties of deoxyguanosine triphosphate triphosphohydrolase from Escherichia coli (1988), J. Biol. Chem., 263, 1494-1499.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.5.1 | 0.001 | - |
dGTP | - |
Escherichia coli |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.5.1 | Co2+ | activation 17% the rate of Mg2+ | Escherichia coli | |
| 3.1.5.1 | Mg2+ | no activity in absence of | Escherichia coli | |
| 3.1.5.1 | Mn2+ | activation 30% the rate of Mg2+ | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.5.1 | 58900 | - |
homotetramer, 4 * 58900 | Escherichia coli |
| 3.1.5.1 | 230000 | - |
gel filtration | Escherichia coli |
| 3.1.5.1 | 230000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.5.1 | dGTP + H2O | Escherichia coli | - |
deoxyguanosine + triphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.5.1 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.5.1 | 16000 fold to homogeneity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.5.1 | dGTP + H2O | - |
Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
| 3.1.5.1 | dGTP + H2O | highly specific for | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
| 3.1.5.1 | dGTP + H2O | triphosphate group required | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
| 3.1.5.1 | dGTP + H2O | no activity on dGDP | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.5.1 | tetramer | SDS-PAGE | Escherichia coli |
| 3.1.5.1 | tetramer | homotetramer, 4 * 58900 | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.5.1 | 55 | 60 | full activity after 1 h | Escherichia coli |
| 3.1.5.1 | 65 | 70 | 90% denatured after 1 h | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.5.1 | 4000 | - |
dGTP | - |
Escherichia coli | |
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