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Literature summary extracted from

  • Vinggaard, A.M.; Hansen, H.S.
    Characterization and partial purification of phospholipase D from human placenta (1995), Biochim. Biophys. Acta, 1258, 169-176.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 EDTA
-
 Homo sapiens
3.1.4.4 phosphatidylinositol 4,5-bisphosphate
-
 Homo sapiens
3.1.4.4 propanolol only hydrolase activity Homo sapiens
3.1.4.4 sphingosine only hydrolase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 N-ethylmaleimide
-
 Homo sapiens
3.1.4.4 oleic acid
-
 Homo sapiens
3.1.4.4 Triton X-100
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.4
-
 phosphatidylcholine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ not required for activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + alcohol Homo sapiens transphosphaditylation phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O Homo sapiens phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 placenta
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.4 -80°C, partially purified, 4 weeks, 8% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + ethanol
-
 Homo sapiens phosphatidylethanol + choline
-
 ?
3.1.4.4 phosphatidylcholine + ethanolamine
-
 Homo sapiens phosphatidylethanolamine + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Homo sapiens 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phospholipid + alcohol transphosphaditylation Homo sapiens phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Homo sapiens phosphatidic acid + alcohol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 7 7.5
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 5.5 8 Hepes buffer stimulates Homo sapiens