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Literature summary extracted from

  • Zwaal, R.F.A.; Roelofsen, B.
    Phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus (1974), Methods Enzymol., 32, 154-161.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 sodium deoxycholate substrate: egg yolk lipoprotein Bacillus cereus

General Stability

EC Number General Stability Organism
3.1.4.3 1,10-phenanthroline, denaturation Bacillus cereus
3.1.4.3 sodium dodecyl sulfate Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 dithiothreitol
-
 Bacillus cereus
3.1.4.3 EDTA
-
 Bacillus cereus
3.1.4.3 o-phenanthroline
-
 Bacillus cereus
3.1.4.3 SDS
-
 Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Mn2+ restores activity after EDTA treatment Bacillus cereus
3.1.4.3 Zn2+ restores activity after EDTA treatment Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 23000
-
 gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Bacillus cereus
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 ammonium sulfate precipitation, ethanol precipitate, protamine sulfate supernatant, gel filtration, ion-exchange chromatography, 450fold, approximately 98% pure Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 1010
-
 substrate: lecithin Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -20°C, 50% glycerol, 6 months, no loss of activity Bacillus cereus
3.1.4.3 20°C, 50% glycerol, 1 week, no significant loss of activity Bacillus cereus
3.1.4.3 20°C, 50% glycerol, after 1 week: 1% loss of activity per day Bacillus cereus
3.1.4.3 4°C, impure preparations, 77% saturated with (NH4)2SO4, stable Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 cardiolipin + H2O
-
 Bacillus cereus phosphatidylglycerophosphate + 1,2-diacylglycerol
-
 ?
3.1.4.3 glucosaminylphosphatidylglycerol + H2O
-
 Bacillus cereus ?
-
 ?
3.1.4.3 phosphatidic acid + H2O
-
 Bacillus cereus ?
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Bacillus cereus phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylglycerol + H2O
-
 Bacillus cereus 1,2-diacylglycerol + sn-glycerol-3-phosphate
-
 ?
3.1.4.3 phosphatidylinositol + H2O
-
 Bacillus cereus inositol monophosphate + diacylglycerol
-
 ?
3.1.4.3 phosphatidylserine + H2O
-
 Bacillus cereus phosphorylserine + diacylglycerol
-
 ?
3.1.4.3 sphingomyelin + H2O
-
 Bacillus cereus ceramide + phosphorylcholine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 70
-
 for 20 min, no significant loss of activity Bacillus cereus