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Literature summary extracted from

  • Berka, R.M.; Vasil, M.L.
    Phospholipase C (heat-labile hemolysin) of Pseudomonas aeruginosa: Purification and preliminary characterization (1982), J. Bacteriol., 152, 239-245.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 76000
-
 gel filtration Pseudomonas aeruginosa
3.1.4.3 78000
-
 SDS-PAGE, gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 ammonium sulfate precipitation, column chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 0.56
-
 substrate: p-nitrophenylphosphorylcholine Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 lysophosphatidylcholine + H2O
-
 Pseudomonas aeruginosa monoacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Pseudomonas aeruginosa 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 sphingomyelin + H2O
-
 Pseudomonas aeruginosa ceramide + phosphorylcholine
-
 ?