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Literature summary extracted from

  • Ivanov, A.; Titibali, R.W.; Kostadinova, S.
    Characterization of a phospholipase C produced by Pseudomaonas fluorescens (1996), New Microbiol., 19, 113-121.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 cetyltrimethylammonium bromide
-
 Pseudomonas fluorescens
3.1.4.3 sodium deoxycholate
-
 Pseudomonas fluorescens
3.1.4.3 Triton X-100
-
 Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Co2+
-
 Pseudomonas fluorescens
3.1.4.3 Cu2+
-
 Pseudomonas fluorescens
3.1.4.3 EDTA
-
 Pseudomonas fluorescens
3.1.4.3 Mn2+
-
 Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Pseudomonas fluorescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Mg2+
-
 Pseudomonas fluorescens
3.1.4.3 Zn2+
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 36000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 ultrafiltration, column chromatography, 792fold Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 63.3
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -20°C or 4°C, 0.3 M triethanolamine or 0.05 M Tris-HCl, pH 7, stable for up to 12 months Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dilauroylphosphatidylcholine + H2O
-
 Pseudomonas fluorescens 1,2-dilaureoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 lysophosphatidylcholine + H2O
-
 Pseudomonas fluorescens monoacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Pseudomonas fluorescens 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Pseudomonas fluorescens phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylinositol + H2O
-
 Pseudomonas fluorescens inositol monophosphate + diacylglycerol
-
 ?
3.1.4.3 sphingomyelin + H2O
-
 Pseudomonas fluorescens ceramide + phosphorylcholine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 6 6.3 substrate: L-a-phosphatidylcholine Pseudomonas fluorescens