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Literature summary extracted from
- The isolation and characterization of sphingomyelinase from human placental tissue (1977), Biochim. Biophys. Acta, 488, 312-321.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.4.12 | albumin stabilizes | Homo sapiens |
| 3.1.4.12 | glycerol, stabilization | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | phosphate | - |
Homo sapiens |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.12 | extracellular | extraction maximized with cutsum | Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 28300 | - |
1 * 36800 + 1 * 28300, SDS-PAGE | Homo sapiens |
| 3.1.4.12 | 36800 | - |
1 * 36800 + 1 * 28300, SDS-PAGE | Homo sapiens |
| 3.1.4.12 | 290000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | 10000fold | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | culture medium | - |
Homo sapiens | - |
| 3.1.4.12 | placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | additional information | - |
different assay conditions, substrates etc. | Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.12 | -20°C, 25 mM citrate buffer, pH 5.9, 2 mg/ml cutsum, 50% glycerol, 5 mM EDTA, 1 mM dithiothreitol, more than 3 months | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | 2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O | - |
Homo sapiens | 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | dimer | 1 * 36800 + 1 * 28300, SDS-PAGE | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 5 | - |
- |
Homo sapiens |
| 3.1.4.12 | 5 | 5.5 | hexadecanoyl-p-nitrophenyl phosphorylcholine | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 3.5 | 6.5 | natural substrate | Homo sapiens |
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Release 2023.1 (January 2023)
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