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Literature summary extracted from

  • Hostetler, K.Y.; Yazaki, P.J.
    The subcellular localization of neutral sphingomyelinase in rat liver (1979), J. Lipid Res., 20, 456-463.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 5'-AMP lysosomes Rattus norvegicus
3.1.4.12 AMP lysosomes Rattus norvegicus
3.1.4.12 Hg2+ plasma membrane and microsomes Rattus norvegicus
3.1.4.12 Mg2+ lysosomal enzyme, inhibition at below pH 4.8 Rattus norvegicus
3.1.4.12 n-butanol plasma membrane Rattus norvegicus
3.1.4.12 p-chloromercuribenzoate plasma membrane and microsomes Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.62
-
 sphingomyelin microsomes Rattus norvegicus
3.1.4.12 1.2
-
 sphingomyelin lysosomes, at 0.5 mM sphingomyelin Rattus norvegicus
3.1.4.12 13
-
 sphingomyelin lysosomes, at 2 mM sphingomyelin Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 cytosol
-
 Rattus norvegicus 5829
-
3.1.4.12 lysosome
-
 Rattus norvegicus 5764
-
3.1.4.12 membrane
-
 Rattus norvegicus 16020
-
3.1.4.12 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Ca2+ lysosomal enzyme, stimulation at pH 5-7.5 Rattus norvegicus
3.1.4.12 Co2+ lysosomal enzyme, stimulation at pH 5-7.5 Rattus norvegicus
3.1.4.12 Mg2+ lysosomal enzyme, stimulation at pH 5-7.5 Rattus norvegicus
3.1.4.12 Mg2+ activation of plasma membrane and microsomal enzyme Rattus norvegicus
3.1.4.12 Mn2+ lysosomal enzyme, stimulation at pH 5-7.5 Rattus norvegicus
3.1.4.12 Mn2+ activation of plasma membrane and microsomal enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 additional information
-
 different assay conditions, substrates etc. Rattus norvegicus
3.1.4.12 additional information
-
 microsomes, lysosomes and plasma membrane Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Rattus norvegicus N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.12 55
-
 plasma membrane and microsomes, 10 min, 15% activity, lysosomes, 10 min, 78% activity Rattus norvegicus
3.1.4.12 70
-
 denaturation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 4.4 4.8 in lysosomes Rattus norvegicus
3.1.4.12 7 7.2 plasma membranes Rattus norvegicus
3.1.4.12 7.2
-
 microsomes Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.12 5 8.5 plasma membranes Rattus norvegicus
3.1.4.12 5 7.5 lysosomal enzyme stimulated by different divalent cations Rattus norvegicus