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Literature summary extracted from

  • Jones, C.S.; Shankaran, P.; Callahan, J.W.
    Purification of sphingomyelinase to apparent homogeneity by using hydrophobic chromatography (1981), Biochem. J., 195, 373-382.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.025
-
 sphingomyelin
-
 Homo sapiens
3.1.4.12 14.5
-
 bis-p-nitrophenyl phosphate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 30700
-
 1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens
3.1.4.12 47500
-
 1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens
3.1.4.12 89100
-
 1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 10000-140000fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 additional information
-
 different assay conditions, substrates etc. Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.4.12 4°C, 0.5% Triton X-100, more than 4 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 bis(4-methylumbelliferyl)-phosphate + H2O
-
 Homo sapiens ?
-
 ?
3.1.4.12 bis-p-nitrophenyl phosphate + H2O
-
 Homo sapiens ?
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 trimer 1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 4.6
-
-
 Homo sapiens
3.1.4.12 4.8 5 bis-4-methylumbelliferyl phosphate and bis-p-nitrophenyl phosphate Homo sapiens