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Literature summary extracted from

  • Anfinsen, C.B; Cuatrecasas, P.; Taniuchi, H.
    Staphylococcal nuclease chemical properties and catalysis (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 177-204.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.31.1 Ca2+ competitive Staphylococcus aureus
3.1.31.1 Cu2+ competitive Staphylococcus aureus
3.1.31.1 Hg2+ competitive Staphylococcus aureus
3.1.31.1 Mononucleotides with 5'-phosphate end group Staphylococcus aureus
3.1.31.1 oligonucleotides with 5'-phosphate end group Staphylococcus aureus
3.1.31.1 Zn2+ competitive Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.31.1 additional information
-
 additional information
-
 Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.31.1 Ca2+ required Staphylococcus aureus
3.1.31.1 Cu2+ minimal activation if Ca2+ is replaced by Cu2+ Staphylococcus aureus
3.1.31.1 Fe2+ minimal activation if Ca2+ is replaced by Fe2+ Staphylococcus aureus
3.1.31.1 Sr2+ DNase but no RNase activity if Ca2+ is replaced Sr2+ Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
-
-
3.1.31.1 Staphylococcus aureus Foggi Worthington
-
-
-
3.1.31.1 Staphylococcus aureus V8
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.31.1 endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products mechanism Staphylococcus aureus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.31.1 high concentration of urea and guanidinium chloride denaturation is completely reversible at pH 3.5-3.7, 55-65°C Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 3'-O-acetylnitrophenyl-pdT + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 5'-chloromethyl-pdTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 5'-O-acetyl-dTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 5'-sulfate-dTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides
-
 ?
3.1.31.1 DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides
-
 ?
3.1.31.1 DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides
-
 ?
3.1.31.1 DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides
-
 ?
3.1.31.1 DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus Foggi Worthington 3'-deoxymononucleotides + dinucleotides
-
 ?
3.1.31.1 DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus Foggi Worthington 3'-deoxymononucleotides + dinucleotides
-
 ?
3.1.31.1 dTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 methyl-pdTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information specificity Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information specificity Staphylococcus aureus V8 ?
-
 ?
3.1.31.1 additional information specificity Staphylococcus aureus Foggi Worthington ?
-
 ?
3.1.31.1 nitrophenyl-pdT + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 nitrophenyl-pdT + H2O
-
 Staphylococcus aureus V8 ?
-
 ?
3.1.31.1 nitrophenyl-pdT + H2O
-
 Staphylococcus aureus Foggi Worthington ?
-
 ?
3.1.31.1 nitrophenyl-pdTp + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 nitrophenyl-pdTpdTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
3.1.31.1 RNA + H2O
-
 Staphylococcus aureus nucleoside 3'-phosphates + dinucleotides
-
 ?
3.1.31.1 RNA + H2O
-
 Staphylococcus aureus V8 nucleoside 3'-phosphates + dinucleotides
-
 ?
3.1.31.1 RNA + H2O
-
 Staphylococcus aureus Foggi Worthington nucleoside 3'-phosphates + dinucleotides
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.31.1 additional information
-
 additional information
-
 Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.31.1 additional information
-
 pH optimum depends on Ca2+ concentration Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.31.1 9 10 depends on Ca2+ concentration Staphylococcus aureus