Any feedback?
Literature summary extracted from
- A comparative study of nucleases exhibiting preference for single-stranded nucleic acid (1986), Biochim. Biophys. Acta, 867, 76-80.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | analysis | - |
Vigna radiata var. radiata |
| 3.1.30.1 | analysis | - |
Neurospora crassa |
| 3.1.30.1 | analysis | - |
Penicillium citrinum |
| 3.1.30.1 | analysis | studies of nucleic acid secondary structure | Aspergillus oryzae |
| 3.1.30.1 | analysis | studies of nucleic acid secondary structure | Schizophyllum commune |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | 8-hydroxychinoline 5-sulfonate | 10% of maximal activity with 0.01 mM, no activity with 0.1 mM | Neurospora crassa |  |
| 3.1.30.1 | 8-hydroxychinoline 5-sulfonate | - |
Pisum sativum | |
| 3.1.30.1 | 8-hydroxychinoline 5-sulfonate | 8% of maximal activity with 0.01 mM, no activity with 0.1 mM | Schizophyllum commune | |
| 3.1.30.1 | 8-hydroxychinoline 5-sulfonate | 30% of maximal activity with 0.01 mM | Vigna radiata var. radiata | |
| 3.1.30.1 | EDTA | - |
Aspergillus oryzae | |
| 3.1.30.1 | EDTA | almost complete inactivation by 0.01 mM | Neurospora crassa | |
| 3.1.30.1 | EDTA | - |
Pisum sativum | |
| 3.1.30.1 | EDTA | 15% of maximal activity with 0.01 mM | Schizophyllum commune | |
| 3.1.30.1 | EDTA | almost complete inactivation by 0.01 mM | Vigna radiata var. radiata | |
| 3.1.30.1 | F- | complete inhibition by 10 mM | Neurospora crassa | |
| 3.1.30.1 | F- | 18% of maximal activity with 10 mM | Schizophyllum commune | |
| 3.1.30.1 | F- | 5% of maximal activity with 10 mM | Vigna radiata var. radiata | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Co2+ | - |
Neurospora crassa | |
| 3.1.30.1 | Co2+ | - |
Aspergillus oryzae | |
| 3.1.30.1 | Co2+ | - |
Ustilago maydis | |
| 3.1.30.1 | Co2+ | - |
Penicillium citrinum | |
| 3.1.30.1 | Co2+ | - |
Schizophyllum commune | |
| 3.1.30.1 | Zn2+ | - |
Vigna radiata var. radiata | |
| 3.1.30.1 | Zn2+ | - |
Triticum aestivum | |
| 3.1.30.1 | Zn2+ | - |
Neurospora crassa | |
| 3.1.30.1 | Zn2+ | - |
Physarum polycephalum | |
| 3.1.30.1 | Zn2+ | - |
Aspergillus oryzae | |
| 3.1.30.1 | Zn2+ | - |
Ustilago maydis | |
| 3.1.30.1 | Zn2+ | - |
Penicillium citrinum | |
| 3.1.30.1 | Zn2+ | - |
Schizophyllum commune | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 32000 | - |
- |
Aspergillus oryzae |
| 3.1.30.1 | 32000 | - |
gel filtration | Physarum polycephalum |
| 3.1.30.1 | 34000 | - |
gel filtration | Schizophyllum commune |
| 3.1.30.1 | 34000 | 35000 | gel filtration | Vigna radiata var. radiata |
| 3.1.30.1 | 34000 | 35000 | gel filtration | Aspergillus oryzae |
| 3.1.30.1 | 34000 | 35000 | gel filtration | Schizophyllum commune |
| 3.1.30.1 | 39000 | - |
- |
Vigna radiata var. radiata |
| 3.1.30.1 | 43000 | - |
- |
Vigna radiata var. radiata |
| 3.1.30.1 | 43000 | - |
gel filtration | Triticum aestivum |
| 3.1.30.1 | 44000 | - |
gel filtration | Penicillium citrinum |
| 3.1.30.1 | 47000 | - |
gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Vigna radiata var. radiata | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Triticum aestivum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Neurospora crassa | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Pisum sativum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Physarum polycephalum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Ustilago maydis | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Penicillium citrinum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Schizophyllum commune | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Aspergillus oryzae | - |
- |
- |
| 3.1.30.1 | Neurospora crassa | - |
- |
- |
| 3.1.30.1 | Penicillium citrinum | - |
- |
- |
| 3.1.30.1 | Physarum polycephalum | - |
- |
- |
| 3.1.30.1 | Pisum sativum | - |
- |
- |
| 3.1.30.1 | Schizophyllum commune | - |
- |
- |
| 3.1.30.1 | Triticum aestivum | - |
- |
- |
| 3.1.30.1 | Ustilago maydis | - |
- |
- |
| 3.1.30.1 | Vigna radiata var. radiata | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Triticum aestivum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Physarum polycephalum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Ustilago maydis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Penicillium citrinum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Schizophyllum commune | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Triticum aestivum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Physarum polycephalum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Ustilago maydis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Penicillium citrinum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Schizophyllum commune | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | mung bean nuclease I | - |
Vigna radiata var. radiata |
| 3.1.30.1 | Neurospora crassa endonuclease | - |
Neurospora crassa |
| 3.1.30.1 | S1 nuclease | - |
Aspergillus oryzae |
| 3.1.30.1 | SC nuclease | - |
Schizophyllum commune |
| 3.1.30.1 | Ustilago maydis nuclease | - |
Ustilago maydis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
