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Literature summary extracted from
- Single-strand-specific nucleases (1982), Cold Spring Harbor Monogr. Ser., 14, 155-185.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | cysteine | 1 mM | Vigna radiata var. radiata |  |
| 3.1.30.1 | glutathione | oxidized form, about 2fold stimulation of activity at all pH | Neurospora crassa | |
| 3.1.30.1 | Triton X-100 | - |
Bacillus subtilis | |
| 3.1.30.1 | Triton X-100 | stabilization of activity at pH 5.0 with 0.001% | Vigna radiata var. radiata | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | analysis | - |
Vigna radiata var. radiata |
| 3.1.30.1 | analysis | - |
Aspergillus oryzae |
| 3.1.30.1 | analysis | analysis of cap structures at the 5'-termini of eucaryotic mRNA | Penicillium citrinum |
| 3.1.30.1 | analysis | gene isolation | Neurospora crassa |
| 3.1.30.1 | synthesis | industrial production of 5'-phosphomononucleotides from yeast RNA | Aspergillus oryzae |
| 3.1.30.1 | synthesis | industrial production of 5'-phosphomononucleotides from yeast RNA | Penicillium citrinum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | 2-mercaptoethanol | with 4 mM 50% of activity with denaturated DNA | Neurospora crassa | |
| 3.1.30.1 | 2-mercaptoethanol | - |
Ustilago maydis | |
| 3.1.30.1 | 5'-ATP | - |
Neurospora crassa | |
| 3.1.30.1 | 5'-ATP | - |
Ustilago maydis | |
| 3.1.30.1 | 5'-dAMP | - |
Aspergillus oryzae | |
| 3.1.30.1 | 5'-dATP | - |
Aspergillus oryzae | |
| 3.1.30.1 | Ca2+ | - |
Homo sapiens | |
| 3.1.30.1 | Ca2+ | - |
Mus musculus | |
| 3.1.30.1 | Ca2+ | about 40% inhibition of hydrolysis of RNA | Neurospora crassa | |
| 3.1.30.1 | CsCl | optimal activity 0.5-1.8 M, 27% of maximal activity at 7 M | Pseudoalteromonas espejiana | |
| 3.1.30.1 | EDTA | complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+ | Aspergillus oryzae | |
| 3.1.30.1 | EDTA | complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+ | Mus musculus | |
| 3.1.30.1 | EDTA | 95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+; complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+ | Neurospora crassa | |
| 3.1.30.1 | EDTA | complete inactivation by incubation with 20 mM, pH 5.0 | Penicillium citrinum | |
| 3.1.30.1 | EDTA | complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+; no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine | Vigna radiata var. radiata | |
| 3.1.30.1 | Fe2+ | - |
Homo sapiens | |
| 3.1.30.1 | Fe2+ | about 40% inhibition of hydrolysis of RNA | Neurospora crassa | |
| 3.1.30.1 | glutathione | with 4 mM of the reduced form 50% of activity with denaturated DNA | Neurospora crassa | |
| 3.1.30.1 | Ionic strength | - |
Neurospora crassa | |
| 3.1.30.1 | Ionic strength | - |
Physarum polycephalum | |
| 3.1.30.1 | Ionic strength | optimal 0.025-0.05 M | Vigna radiata var. radiata | |
| 3.1.30.1 | Mg2+ | - |
Mus musculus | |
| 3.1.30.1 | Mg2+ | about 40% inhibition of hydrolysis of RNA with 10 mM Mg2+ | Neurospora crassa | |
| 3.1.30.1 | NaCl | - |
Aspergillus oryzae | |
| 3.1.30.1 | NaCl | - |
Homo sapiens | |
| 3.1.30.1 | NaCl | - |
Mus musculus | |
| 3.1.30.1 | NaCl | maximal activity 0-2 M, 40% of maximal activity at 4,4 M | Pseudoalteromonas espejiana | |
| 3.1.30.1 | phosphate | - |
Aspergillus oryzae | |
| 3.1.30.1 | phosphate | - |
Mus musculus | |
| 3.1.30.1 | phosphate | 80% inhibition by 33 mM potassium phosphate, pH 7.5 | Neurospora crassa | |
| 3.1.30.1 | phosphate | competitive inhibition | Physarum polycephalum | |
| 3.1.30.1 | phosphate | - |
Ustilago maydis | |
| 3.1.30.1 | sodium dodecylsulfate | up to 0.6% no effect on DNA hydrolysis | Aspergillus oryzae | |
| 3.1.30.1 | sodium dodecylsulfate | - |
Mus musculus | |
| 3.1.30.1 | sodium dodecylsulfate | complete inactivation with 0.01% at pH 5.0 | Vigna radiata var. radiata | |
| 3.1.30.1 | Urea | 40% of maximal activity with single-stranded DNA with 6.6 M | Pseudoalteromonas espejiana | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Ca2+ | 2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM | Neurospora crassa | |
| 3.1.30.1 | Co2+ | - |
Mus musculus | |
| 3.1.30.1 | Co2+ | - |
Aspergillus oryzae | |
| 3.1.30.1 | Co2+ | - |
Ustilago maydis | |
| 3.1.30.1 | Co2+ | - |
Penicillium citrinum | |
| 3.1.30.1 | Co2+ | up to 3fold stimulation of DNA hydrolysis rate by 0.5 mM | Neurospora crassa | |
| 3.1.30.1 | Fe2+ | 2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM | Neurospora crassa | |
| 3.1.30.1 | Mg2+ | - |
Mus musculus | |
| 3.1.30.1 | Mg2+ | - |
Homo sapiens | |
| 3.1.30.1 | Mg2+ | - |
Mustelus canis | |
| 3.1.30.1 | Mg2+ | hydrolysis of single-stranded DNA in the absence of Mg2+ at 1.5% of the rate at 15 mM Mg2+ | Pseudoalteromonas espejiana | |
| 3.1.30.1 | Mg2+ | 2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM | Neurospora crassa | |
| 3.1.30.1 | Mn2+ | - |
Aspergillus oryzae | |
| 3.1.30.1 | Mn2+ | - |
Mustelus canis | |
| 3.1.30.1 | Zn2+ | - |
Vigna radiata var. radiata | |
| 3.1.30.1 | Zn2+ | - |
Triticum aestivum | |
| 3.1.30.1 | Zn2+ | - |
Neurospora crassa | |
| 3.1.30.1 | Zn2+ | - |
Physarum polycephalum | |
| 3.1.30.1 | Zn2+ | - |
Ustilago maydis | |
| 3.1.30.1 | Zn2+ | enzyme contains 3 zinc atoms | Aspergillus oryzae | |
| 3.1.30.1 | Zn2+ | enzyme contains 3 zinc atoms | Penicillium citrinum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 32000 | - |
- |
Physarum polycephalum |
| 3.1.30.1 | 32000 | - |
- |
Aspergillus oryzae |
| 3.1.30.1 | 39000 | - |
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active | Vigna radiata var. radiata |
| 3.1.30.1 | 42000 | 50000 | - |
Penicillium citrinum |
| 3.1.30.1 | 54000 | - |
- |
Homo sapiens |
| 3.1.30.1 | 55000 | - |
- |
Neurospora crassa |
| 3.1.30.1 | 57000 | - |
- |
Bacillus subtilis |
| 3.1.30.1 | 90000 | - |
single inactive precursor polypeptide, generation of four nucleases by proteolysis: 75000: Mg2+ dependent exonuclease, 65000: endo-exonuclease, 55000: single-strand-specific endonuclease, 65000: Dnase A, a Ca2+ dependent endonuclease | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Vigna radiata var. radiata | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Bacillus subtilis | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Mus musculus | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Homo sapiens | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Triticum aestivum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Neurospora crassa | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Physarum polycephalum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Ustilago maydis | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Penicillium citrinum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Mustelus canis | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Pseudoalteromonas espejiana | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Vigna radiata var. radiata | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Mus musculus | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Triticum aestivum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Neurospora crassa | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Physarum polycephalum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Ustilago maydis | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | Penicillium citrinum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Aspergillus oryzae | - |
- |
- |
| 3.1.30.1 | Bacillus subtilis | - |
Marburg strain | - |
| 3.1.30.1 | Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | - |
Marburg strain | - |
| 3.1.30.1 | Escherichia coli | - |
- |
- |
| 3.1.30.1 | Homo sapiens | - |
- |
- |
| 3.1.30.1 | Mus musculus | - |
- |
- |
| 3.1.30.1 | Mustelus canis | - |
- |
- |
| 3.1.30.1 | Neurospora crassa | - |
- |
- |
| 3.1.30.1 | Penicillium citrinum | - |
- |
- |
| 3.1.30.1 | Physarum polycephalum | - |
- |
- |
| 3.1.30.1 | Pseudoalteromonas espejiana | - |
- |
- |
| 3.1.30.1 | Triticum aestivum | - |
- |
- |
| 3.1.30.1 | Ustilago maydis | - |
- |
- |
| 3.1.30.1 | Vigna radiata var. radiata | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | glycoprotein | - |
Aspergillus oryzae |
| 3.1.30.1 | glycoprotein | 29% carbohydrate by weight | Vigna radiata var. radiata |
| 3.1.30.1 | glycoprotein | 17.4% carbohydrate consisting of mannose, galactose, glucosamine in a ratio of 6/2/1 | Penicillium citrinum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | ascites tumor cell | - |
Mus musculus | - |
| 3.1.30.1 | plasmodium | microplasmodium | Physarum polycephalum | - |
| 3.1.30.1 | seedling | germinating | Triticum aestivum | - |
| 3.1.30.1 | sprout | - |
Vigna radiata var. radiata | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.30.1 | 4°C, crude and purified form, no loss of activity after several years | Penicillium citrinum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | 3'-AMP + H2O | - |
Vigna radiata var. radiata | adenosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-AMP + H2O | - |
Aspergillus oryzae | adenosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-AMP + H2O | - |
Penicillium citrinum | adenosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-CMP + H2O | - |
Vigna radiata var. radiata | cytosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-CMP + H2O | - |
Aspergillus oryzae | cytosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-CMP + H2O | - |
Penicillium citrinum | cytosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dAMP + H2O | - |
Vigna radiata var. radiata | 3'-deoxyadenosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dAMP + H2O | - |
Aspergillus oryzae | 3'-deoxyadenosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dCMP + H2O | - |
Vigna radiata var. radiata | deoxycytosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dCMP + H2O | - |
Aspergillus oryzae | deoxycytosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dGMP + H2O | - |
Vigna radiata var. radiata | deoxyguanosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dGMP + H2O | - |
Aspergillus oryzae | deoxyguanosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dTMP + H2O | - |
Vigna radiata var. radiata | deoxythymidine + phosphate | - |
? | |
| 3.1.30.1 | 3'-dTMP + H2O | - |
Aspergillus oryzae | deoxythymidine + phosphate | - |
? | |
| 3.1.30.1 | 3'-GMP + H2O | - |
Vigna radiata var. radiata | guanosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-GMP + H2O | - |
Aspergillus oryzae | guanosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-GMP + H2O | - |
Penicillium citrinum | guanosine + phosphate | - |
? | |
| 3.1.30.1 | 3'-UMP + H2O | - |
Vigna radiata var. radiata | uridine + phosphate | - |
? | |
| 3.1.30.1 | 3'-UMP + H2O | - |
Aspergillus oryzae | uridine + phosphate | - |
? | |
| 3.1.30.1 | 3'-UMP + H2O | - |
Penicillium citrinum | uridine + phosphate | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Bacillus subtilis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Triticum aestivum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Physarum polycephalum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Ustilago maydis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Penicillium citrinum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Mustelus canis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Pseudoalteromonas espejiana | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | only 2% of activity on ssDNA | Bacillus subtilis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA | Pseudoalteromonas espejiana | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | only 2% of activity on ssDNA | Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyadenylic acid + H2O | - |
Penicillium citrinum | 5'-AMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyadenylic acid + H2O | only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C, at pH 6.4 50% of activity in comparision with DNA | Aspergillus oryzae | 5'-AMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polycytosylic acid + H2O | - |
Penicillium citrinum | 5'-CMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polycytosylic acid + H2O | only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C, at pH 6.4 50% of activity in comparision with DNA | Aspergillus oryzae | 5'-CMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyguanylic acid + H2O | only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C | Aspergillus oryzae | 5'-GMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyinosinic acid + H2O | - |
Penicillium citrinum | 5'-IMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyuridylic acid + H2O | - |
Homo sapiens | 5'-UMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyuridylic acid + H2O | - |
Penicillium citrinum | 5'-UMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyuridylic acid + H2O | activity comparable with denaturated DNA at pH 4.6, 0.05 M NaCl and 45°C | Aspergillus oryzae | 5'-UMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Triticum aestivum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Physarum polycephalum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Ustilago maydis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | RNA + H2O | - |
Penicillium citrinum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Bacillus subtilis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Triticum aestivum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Physarum polycephalum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Ustilago maydis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Penicillium citrinum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Pseudoalteromonas espejiana | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | from 5'-phosphomononucleotides to at least 5'-phosphoheptanucleotides | ? | |
| 3.1.30.1 | ssDNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | mainly 5'-phosphooligonucleotides with 5-10 residues | ? | |
| 3.1.30.1 | ssDNA + H2O | 15 times more active towards thermally denaturated than native DNA | Mustelus canis | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | specificity for phosphodiester bonds adjoining deoxycytidine | Escherichia coli | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | 5'-phosphooligonucleotides with average chain lengh of about 50 | ? | |
| 3.1.30.1 | ssDNA + H2O | - |
Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | Alteromonas BAL 31 nuclease | - |
Pseudoalteromonas espejiana |
| 3.1.30.1 | Bacillus subtilis deoxyribonuclease | - |
Bacillus subtilis |
| 3.1.30.1 | mung bean nuclease I | - |
Vigna radiata var. radiata |
| 3.1.30.1 | Neurospora crassa nuclease | - |
Neurospora crassa |
| 3.1.30.1 | P1 nuclease | - |
Penicillium citrinum |
| 3.1.30.1 | Phage T4 endonuclease IV | - |
Escherichia coli |
| 3.1.30.1 | Physarum polycephalum nuclease | - |
Physarum polycephalum |
| 3.1.30.1 | single-strand-specific nuclease | - |
Aspergillus oryzae |
| 3.1.30.1 | wheat seedling nuclease | - |
Triticum aestivum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 30 | 60 | optimal temperature for denaturated DNA 60°C, 13% of maximal activity at 30°C | Pseudoalteromonas espejiana |
| 3.1.30.1 | 47 | 62 | maximal hydrolysis rate of denaturated DNA and RNA | Neurospora crassa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 60 | 70 | stable to heating at pH 5.0 in the presence of both Zn2+ and sulfhydryl compounds | Vigna radiata var. radiata |
| 3.1.30.1 | 60 | 67 | stable below 60°C, 50% inactivation after heating at 67°C for 15 min, pH 6.0 | Penicillium citrinum |
| 3.1.30.1 | 65 | - |
no inactivation of ribonuclease and deoxyribonuclease activity by heating at this temperature in the presence of substrate | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 4.5 | 5 | with denaturated DNA in the presence of 1 mM Zn2+ | Aspergillus oryzae |
| 3.1.30.1 | 5 | - |
- |
Vigna radiata var. radiata |
| 3.1.30.1 | 5 | - |
with natural single-stranded DNA and RNA as substrate | Penicillium citrinum |
| 3.1.30.1 | 7.5 | 8.5 | 0.1-0.2 M Tris-HCl buffer | Neurospora crassa |
| 3.1.30.1 | 8 | - |
- |
Physarum polycephalum |
| 3.1.30.1 | 8 | - |
optimal in 0.1 M NaCl | Mustelus canis |
| 3.1.30.1 | 8 | 8.8 | - |
Pseudoalteromonas espejiana |
| 3.1.30.1 | 9.2 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 4 | 6 | with synthetic homopolynucleotides as substrate | Penicillium citrinum |
| 3.1.30.1 | 5.8 | 9.3 | - |
Aspergillus oryzae |
| 3.1.30.1 | 5.8 | 9.3 | 50% of activity at pH 5.8 and 9.3 | Neurospora crassa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 4.8 | 5.5 | Zn2+ essential for stabilizing activity | Triticum aestivum |
| 3.1.30.1 | 5 | 8 | - |
Penicillium citrinum |
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Release 2023.1 (January 2023)
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