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Literature summary extracted from
- Specificity of the S1 nuclease from Aspergillus oryzae (1975), J. Biol. Chem., 250, 8848-8855.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | analysis | analysis of heteroduplex and superhelical DNA, quantitation of nucleic acid hybridization | Aspergillus oryzae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | 5'-dAMP | 50% inhibition by 0.085 mM dAMP | Aspergillus oryzae |  |
| 3.1.30.1 | 5'-dATP | 50% inhibition by 0.001 mM dATP | Aspergillus oryzae | |
| 3.1.30.1 | phosphate | 50% inhibition by 2 mM sodium phosphate | Aspergillus oryzae | |
| 3.1.30.1 | sodium bisphosphate | 50% inhibition by 0.02 mM sodium bisphosphate | Aspergillus oryzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Co2+ | - |
Aspergillus oryzae | |
| 3.1.30.1 | Hg2+ | maximal activity at 0.01-1 mM at pH 3.5-5.0 | Aspergillus oryzae | |
| 3.1.30.1 | Zn2+ | - |
Aspergillus oryzae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Aspergillus oryzae | 75000 times more active on single-stranded than on native DNA | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.1 | - |
Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 12.7 | - |
purified enzyme, heat-denaturated DNA as substrate | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | 75000 times more active on single-stranded than on native DNA | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | S1 nuclease | - |
Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 3.5 | 5 | - |
Aspergillus oryzae |
| 3.1.30.1 | 4.5 | - |
in 10 mM NaCl | Aspergillus oryzae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.30.1 | 0.001 | - |
5'-dATP | - |
Aspergillus oryzae | |
| 3.1.30.1 | 0.085 | - |
5'-dAMP | - |
Aspergillus oryzae | |
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Release 2023.1 (January 2023)
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