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Literature summary extracted from

  • Gold, E.W.; Veitch, F.P.
    Studies on a pyrophosphatase and glucose-6-phosphatase from Aspergillus oryzae (1973), Biochim. Biophys. Acta, 327, 166-175.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.9 molybdate
-
Aspergillus oryzae
3.6.1.1 D-glucose non-competitive Aspergillus oryzae
3.6.1.1 D-Glucose-6-phosphate competitive Aspergillus oryzae
3.6.1.1 MoO42-
-
Aspergillus oryzae
3.6.1.1 Zn2+
-
Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.9 0.51
-
glucose 6-phosphate
-
Aspergillus oryzae
3.6.1.1 0.55
-
diphosphate pH 3 Aspergillus oryzae
3.6.1.1 1.32
-
diphosphate pH 6.9 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.9 60800
-
gel filtration Aspergillus oryzae
3.6.1.1 60800
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Aspergillus oryzae
-
2 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.9 Aspergillus oryzae
-
-
-
3.6.1.1 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.9
-
Aspergillus oryzae
3.6.1.1
-
Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 13.1
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.9 D-glucose 6-phosphate + H2O
-
Aspergillus oryzae D-glucose + phosphate
-
?
3.1.3.9 additional information activities for EC 3.1.3.9, EC 3.6.1.1, and pyrophosphate-glucose phosphotransferase are due to a single enzyme Aspergillus oryzae ?
-
?
3.6.1.1 diphosphate + H2O
-
Aspergillus oryzae 2 phosphate
-
?
3.6.1.1 additional information enzyme may be identical with EC 3.1.3.1 or EC 3.1.3.9 Aspergillus oryzae ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 60
-
50% loss of activity after 30 min Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.9 5.7
-
-
Aspergillus oryzae
3.6.1.1 3
-
-
Aspergillus oryzae
3.6.1.1 6.1 6.2
-
Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.9 4.5
-
stable Aspergillus oryzae
3.6.1.1 4.5
-
-
Aspergillus oryzae