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Literature summary extracted from

  • Anchors, J.M.; Karnovsky, M.L.
    Purification of cerebral glucose-6-phosphatase. An enzyme involved in sleep (1975), J. Biol. Chem., 250, 6408-6416.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.9 sodium barbital 3 mM, complete inhibition Rattus norvegicus
3.1.3.9 sodium pentobarbital 3 mM, complete inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.9 microsome
-
 Rattus norvegicus
-
-
3.1.3.9 nucleus
-
 Rattus norvegicus 5634
-
3.1.3.9 plasma membrane
-
 Rattus norvegicus 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.9 28000
-
 enzyme extracted with 10 mM Tris, pH 8.5, 1.0% sodium deoxycholate w/v, gel filtration Rattus norvegicus
3.1.3.9 70000
-
 enzyme extracted with 10 mM sodium phosphate, pH 7.0, with 1.0% sodium deoxycholate and filtered on a Sephadex column equilibrated with the neutral buffer containing 0.1% detergent, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.9 additional information Rattus norvegicus the enzyme is involved in sleep ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.9
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.9 brain equally distributed in all areas of the brain Rattus norvegicus
-
3.1.3.9 brain the enzyme is found in all parts of the brain in the membraneous subcellular fractions of neurons Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.9 7.85
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.3.9 -30°C, not stable for more than a few days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.9 5'-AMP + H2O 2.6% of the activity with D-glucose 6-phosphate Rattus norvegicus ?
-
 ?
3.1.3.9 beta-glycerophosphate + H2O 1.2% of the activity with D-glucose 6-phosphate Rattus norvegicus glycerol + phosphate
-
 ?
3.1.3.9 D-glucose 6-phosphate + H2O
-
 Rattus norvegicus D-glucose + phosphate
-
 ?
3.1.3.9 diphosphate + H2O 23% of the activity with D-glucose 6-phosphate Rattus norvegicus 2 phosphate
-
 ?
3.1.3.9 additional information the enzyme is involved in sleep Rattus norvegicus ?
-
 ?
3.1.3.9 p-nitrophenylphosphate + H2O 1.4% of the activity with D-glucose 6-phosphate Rattus norvegicus p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.9 ? x * 28000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.9 21
-
 inactivated at room temperature Rattus norvegicus
3.1.3.9 45
-
 10 min, 80% loss of activity Rattus norvegicus