EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.4 | Triton X-100 | the 45000 Da enzyme form shows maximal activity at 5 mM | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.4 | NEM | 45000 Da enzyme form, at 1 mM | Saccharomyces cerevisiae | |
3.1.3.4 | p-chloromercuriphenylsulfonic acid | 45000 Da enzyme form, at 1 mM | Saccharomyces cerevisiae | |
3.1.3.4 | Phenylglyoxal | inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form | Saccharomyces cerevisiae | |
3.1.3.4 | propanolol | inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.4 | microsome | - |
Saccharomyces cerevisiae | - |
- |
3.1.3.4 | mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
3.1.3.4 | plasma membrane | associated | Saccharomyces cerevisiae | 5886 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.4 | Mg2+ | required for the 45000 Da enzyme form, maximal activity at 1 mM | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.4 | additional information | Saccharomyces cerevisiae | the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.4 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.4 | 45000 Da enzyme form and 104000 Da enzyme form | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.4 | 2.35 | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.3.4 | -80°C, the 45000 Da enzyme form is stable for 3 months, the 104000 Da enzyme form is degraded to a 91000 Da enzyme form and retains full activity | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.4 | additional information | the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth | Saccharomyces cerevisiae | ? | - |
? | |
3.1.3.4 | phosphatidic acid + H2O | - |
Saccharomyces cerevisiae | 1,2-diacyl-sn-glycerol + phosphate | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.4 | 30 | - |
45000 Da enzyme form, unstable above | Saccharomyces cerevisiae |
3.1.3.4 | 50 | - |
45000 Da enzyme form, complete inactivation after 20 min | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.4 | 6 | 7 | 45000 Da enzyme form | Saccharomyces cerevisiae |