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Literature summary extracted from

  • Chaimovich, H.; Nome, F.
    Purification and properties of an acid phosphatase from bovine brain (1970), Arch. Biochem. Biophys., 139, 9-16.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Hg2+
-
 Bos taurus
3.1.3.2 Mn2+
-
 Bos taurus
3.1.3.2 NEM
-
 Bos taurus
3.1.3.2 p-Nitrophenyl sulfate inhibits hydrolysis of p-nitrophenyl phosphate Bos taurus
3.1.3.2 PCMB
-
 Bos taurus
3.1.3.2 phosphate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.07
-
 p-nitrophenyl phosphate
-
 Bos taurus
3.1.3.2 0.075
-
 p-nitrophenyl phosphate
-
 Bos taurus
3.1.3.2 1
-
 alpha-glycerophosphate
-
 Bos taurus
3.1.3.2 1.4
-
 o-nitrophenyl phosphate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Zn2+
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 brain
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 ADP + H2O no activity Bos taurus AMP + phosphate
-
 ?
3.1.3.2 alpha-glycerophosphate + H2O 15% of the activity with p-nitrophenylphosphate Bos taurus glycerol + phosphate
-
 ?
3.1.3.2 AMP + H2O no activity Bos taurus adenosine + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O no activity Bos taurus D-fructose + phosphate
-
 ?
3.1.3.2 dAMP + H2O 5% of the activity with p-nitrophenylphosphate Bos taurus deoxyadenosine + phosphate
-
 ?
3.1.3.2 diphosphate + H2O no activity Bos taurus 2 phosphate
-
 ?
3.1.3.2 glucose 1-phosphate + H2O no activity Bos taurus glucose + phosphate
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O no activity Bos taurus Glucose + phosphate
-
 ?
3.1.3.2 GMP + H2O no activity Bos taurus guanosine + phosphate
-
 ?
3.1.3.2 o-nitrophenyl phosphate + H2O 21% of the activity with p-nitrophenylphosphate Bos taurus o-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Bos taurus p-nitrophenol + phosphate
-
 ?
3.1.3.2 pyridoxal 5'-phosphate + H2O 5% of the activity with p-nitrophenylphosphate Bos taurus pyridoxal + phosphate
-
 ?
3.1.3.2 UMP + H2O 10% of the activity with p-nitrophenylphosphate Bos taurus uridine + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 37
-
 10 min, pH 3.9-6.8, stable Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.8 5.8
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 3.9 6.8 37°C, 10 min, stable Bos taurus