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Literature summary extracted from

  • Tominaga, N.; Mori, T.
    A sulfate-dependent acid phosphatase of Thiobacillus thiooxidans. Its partial purification and some properties (1974), J. Biochem., 76, 397-408.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 arsenate
-
 Acidithiobacillus thiooxidans
3.1.3.2 ascorbate
-
 Acidithiobacillus thiooxidans
3.1.3.2 Cys
-
 Acidithiobacillus thiooxidans
3.1.3.2 dithiothreitol
-
 Acidithiobacillus thiooxidans
3.1.3.2 F- NaF Acidithiobacillus thiooxidans
3.1.3.2 molybdate
-
 Acidithiobacillus thiooxidans
3.1.3.2 NaBH4
-
 Acidithiobacillus thiooxidans
3.1.3.2 phosphate
-
 Acidithiobacillus thiooxidans
3.1.3.2 sodium lauryl sulfate
-
 Acidithiobacillus thiooxidans
3.1.3.2 Tartrate 20 mM, 16% inhibition Acidithiobacillus thiooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.21
-
 p-nitrophenyl phosphate
-
 Acidithiobacillus thiooxidans
3.1.3.2 0.23
-
 alpha-glycerophosphate
-
 Acidithiobacillus thiooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 130000
-
 gel filtration Acidithiobacillus thiooxidans

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Acidithiobacillus thiooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Acidithiobacillus thiooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 additional information
-
-
 Acidithiobacillus thiooxidans

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 0°C, 0.25 M ammonium sulfate, stable for more than 1 month Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2-glycerophosphate + H2O
-
 Acidithiobacillus thiooxidans glycerol + phosphate
-
 ?
3.1.3.2 3'-AMP + H2O
-
 Acidithiobacillus thiooxidans adenosine + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Acidithiobacillus thiooxidans adenosine + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Acidithiobacillus thiooxidans AMP + phosphate
-
 ?
3.1.3.2 alpha-glycerophosphate + H2O
-
 Acidithiobacillus thiooxidans glycerol + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Acidithiobacillus thiooxidans ADP + phosphate
-
 ?
3.1.3.2 D-fructose 1,6-diphosphate + H2O
-
 Acidithiobacillus thiooxidans ?
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O
-
 Acidithiobacillus thiooxidans D-fructose + phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O
-
 Acidithiobacillus thiooxidans D-ribose + phosphate
-
 ?
3.1.3.2 diphosphate + H2O
-
 Acidithiobacillus thiooxidans 2 phosphate
-
 ?
3.1.3.2 glucose 1-phosphate + H2O
-
 Acidithiobacillus thiooxidans glucose + phosphate
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O
-
 Acidithiobacillus thiooxidans Glucose + phosphate
-
 ?
3.1.3.2 GTP + H2O
-
 Acidithiobacillus thiooxidans GDP + phosphate
-
 ?
3.1.3.2 O-phosphoethanolamine + H2O
-
 Acidithiobacillus thiooxidans ethanolamine + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Acidithiobacillus thiooxidans p-nitrophenol + phosphate
-
 ?
3.1.3.2 phosphocholine + H2O
-
 Acidithiobacillus thiooxidans choline + phosphate
-
 ?
3.1.3.2 phosphopyruvate + H2O
-
 Acidithiobacillus thiooxidans pyruvate + phosphate
-
 ?