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Literature summary extracted from

  • Ullah, A.H.J.; Gibson, D.M.
    Purification and characterization of acid phosphatase from cotyledons of germinating soybean seeds (1988), Arch. Biochem. Biophys., 260, 514-520.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.2 50-60% loss of activity after two freeze-thaw cycles Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 phosphate competitive Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.07
-
 p-nitrophenyl phosphate
-
 Glycine max
3.1.3.2 0.085
-
 Na2H2P2O7
-
 Glycine max
3.1.3.2 0.125
-
 ATP
-
 Glycine max
3.1.3.2 0.3
-
 Phenyl phosphate
-
 Glycine max
3.1.3.2 0.4
-
 2-glycerophosphate
-
 Glycine max
3.1.3.2 1
-
 bis(p-nitrophenyl)phosphate
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 53000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 additional information
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 0°C, stable for at least 4 weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2-glycerophosphate + H2O
-
 Glycine max glycerol + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Glycine max ADP + phosphate
-
 ?
3.1.3.2 bis(p-nitrophenyl) phosphate + H2O
-
 Glycine max ?
-
 ?
3.1.3.2 Na2H2P2O7 + H2O
-
 Glycine max ?
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Glycine max p-nitrophenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Glycine max phenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 53000, SDS-PAGE Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 60
-
-
 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 68
-
 10 min, complete loss of activity Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 125
-
 2-glycerophosphate
-
 Glycine max
3.1.3.2 188
-
 ATP
-
 Glycine max
3.1.3.2 219
-
 Phenyl phosphate
-
 Glycine max
3.1.3.2 232
-
 Na2H2P2O7
-
 Glycine max
3.1.3.2 430
-
 p-nitrophenyl phosphate
-
 Glycine max
3.1.3.2 700
-
 bis(p-nitrophenyl) phosphate
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
 Glycine max