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Literature summary extracted from

  • Joh, T.; Yazaki, J.; Suzuki, K.; Hayakawa, T.
    Isolation and properties of glucose-1-phosphatase from mycelia of Pholiota nemako (1998), Biosci. Biotechnol. Biochem., 62, 2251-2253.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.10 (NH4)6Mo7O24 98% inhibition of 4 -nitrophenyl phosphate hydrolysis by 1.0 mM Pholiota nameko
3.1.3.10 CuSO4 55% inhibition of 4-nitrophenyl phosphate hydrolysis by 10 mM Pholiota nameko
3.1.3.10 HgCl2 99% inhibition of 4-nitrophenyl phosphate hydrolysis by 10 mM Pholiota nameko
3.1.3.10 NaF no inhibition through NaF for the acid phosphatase AP35 Pholiota nameko
3.1.3.10 tartaric acid 36% inhibition of 4-nitrophenyl phosphate hydrolysis by 10 mM Pholiota nameko

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.10 2.2
-
 4-nitrophenyl phosphate
-
 Pholiota nameko
3.1.3.10 4.8
-
 D-glucose 1-phosphate
-
 Pholiota nameko
3.1.3.10 7.9
-
 D-ribose 5-phosphate
-
 Pholiota nameko
3.1.3.10 12.2
-
 D-Fructose 1-phosphate
-
 Pholiota nameko
3.1.3.10 12.3
-
 phosphoenolpyruvate
-
 Pholiota nameko
3.1.3.10 13.4
-
 Adenosine diphosphate
-
 Pholiota nameko
3.1.3.10 14.3
-
 D-fructose 6-phosphate
-
 Pholiota nameko
3.1.3.10 56.7
-
 Adenosine triphosphate
-
 Pholiota nameko
3.1.3.10 85.4
-
 D-galactose 1-phosphate
-
 Pholiota nameko

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.10 CaCl2 relative activity in presence of CaCl2 10mM 123% Pholiota nameko
3.1.3.10 EDTA relative activity in presence of EDTA 10mM 104% Pholiota nameko
3.1.3.10 NaF relative activity in presence of NaF 10mM 114% Pholiota nameko

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.10 35000
-
 SDS-PAGE Pholiota nameko

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.10 D-fructose 1-phosphate + H2O Pholiota nameko
-
 D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 6-phosphate + H2O Pholiota nameko
-
 D-fructose + phosphate
-
 ?
3.1.3.10 D-galactose 1-phosphate + H2O Pholiota nameko
-
 D-galactose + phosphate
-
 ?
3.1.3.10 D-glucose 1-phosphate + H2O Pholiota nameko
-
 D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O Pholiota nameko
-
 D-glucose + phosphate
-
 ?
3.1.3.10 D-ribose 5-phosphate + H2O Pholiota nameko
-
 D-ribose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.10 Pholiota nameko
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.10
-
 Pholiota nameko

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.10 30
-
 with ribose 5-phosphate as substrate, purification procedure described Pholiota nameko
3.1.3.10 30
-
 with nitrophenyl phosphate as substrate, purification procedure described Pholiota nameko
3.1.3.10 40
-
 with phosphoenolpyruvate as substrate Pholiota nameko
3.1.3.10 40
-
 purification procedure described Pholiota nameko
3.1.3.10 100
-
 with D-fructose 6-phosphate as substrate, purification procedure described Pholiota nameko
3.1.3.10 130
-
 purification procedure described Pholiota nameko
3.1.3.10 130
-
 with D-fructose 1-phosphate as substrate Pholiota nameko
3.1.3.10 210
-
 purification procedure described Pholiota nameko
3.1.3.10 210
-
 with adenosine diphosphate as substrate Pholiota nameko
3.1.3.10 410
-
 purification procedure described Pholiota nameko
3.1.3.10 410
-
 with adenosine triphosphate as substrate Pholiota nameko
3.1.3.10 2090
-
 purification procedure described Pholiota nameko
3.1.3.10 2090
-
 with D-galactose 1-phosphate as substrate Pholiota nameko
3.1.3.10 2360
-
 purification procedure described Pholiota nameko
3.1.3.10 2360
-
 with D-glucose 1-phosphate as substrate Pholiota nameko

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.10 4-nitrophenyl phosphate + H2O
-
 Pholiota nameko 4-nitrophenol + phosphate
-
 ?
3.1.3.10 ADP + H2O
-
 Pholiota nameko AMP + phosphate
-
 ?
3.1.3.10 ATP + H2O
-
 Pholiota nameko ADP + phosphate
-
 ?
3.1.3.10 D-fructose 1-phosphate + H2O
-
 Pholiota nameko D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 6-phosphate + H2O
-
 Pholiota nameko D-fructose + phosphate
-
 ?
3.1.3.10 D-galactose 1-phosphate + H2O
-
 Pholiota nameko D-galactose + phosphate
-
 ?
3.1.3.10 D-glucose 1-phosphate + H2O
-
 Pholiota nameko D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O
-
 Pholiota nameko D-glucose + phosphate
-
 ?
3.1.3.10 D-ribose 5-phosphate + H2O
-
 Pholiota nameko D-ribose + phosphate
-
 ?
3.1.3.10 phosphoenolpyruvate + H2O
-
 Pholiota nameko pyruvate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.10 acid phosphatase
-
 Pholiota nameko

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.10 40
-
 with 4-nitrophenyl phosphate as substrate Pholiota nameko

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.10 0.0005
-
 ribose 5-phosphate
-
 Pholiota nameko
3.1.3.10 0.000667
-
 phosphoenolpyruvate
-
 Pholiota nameko
3.1.3.10 0.00167
-
 fructose 6-phosphate
-
 Pholiota nameko
3.1.3.10 0.00217
-
 fructose 1-phosphate
-
 Pholiota nameko
3.1.3.10 0.0035
-
 ADP
-
 Pholiota nameko
3.1.3.10 0.0348
-
 Galactose 1-phosphate
-
 Pholiota nameko
3.1.3.10 0.0393
-
 glucose 1-phosphate
-
 Pholiota nameko

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.10 5.9
-
 purified enzyme Pholiota nameko
3.1.3.10 5.9
-
 with 4-nitrophenyl phosphate 15 mM, as substrate Pholiota nameko

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.10 3 7 purified enzyme, with 4-nitrophenyl phosphate 15 mM as substrate, activity rapidly lost above pH8 Pholiota nameko