BRENDA - Enzyme Database

Acid phosphatases of Escherichia coli: molecular cloning and analysis of agp, the structural gene for a periplasmic acid glucose phosphatase

Pradel, E.; Boquet, P.L.; J. Bacteriol. 170, 4916 (1988)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.10
in vivo cloning technique with plasmid Mu dII4042, expression in Escherichia coli strain SBS1295
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.10
0.5
-
D-glucose 1-phosphate
-
Escherichia coli
3.1.3.10
15
-
D-glucose 6-phosphate
-
Escherichia coli
3.1.3.10
25
-
D-fructose 6-phosphate
-
Escherichia coli
3.1.3.10
30
-
4-nitrophenyl phosphate
-
Escherichia coli
3.1.3.10
30
-
D-ribose 5-phosphate
-
Escherichia coli
3.1.3.10
50
-
D-Fructose 1-phosphate
-
Escherichia coli
3.1.3.10
100
-
Glycerol 2-phosphate
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.10
periplasm
periplasm extracts made by osmotic shock of strain SBS1405(pEP1376)
Escherichia coli
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.10
44000
-
SDS-Page of osmotic shock fluid of strain SBS1405(pEP1376)
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.10
D-fructose 1,6-diphosphate + H2O
Escherichia coli
-
D-fructose + phosphate
-
Escherichia coli
-
3.1.3.10
D-fructose 1-phosphate + H2O
Escherichia coli
-
D-fructose + phosphate
-
Escherichia coli
-
3.1.3.10
D-fructose 6-phosphate + H2O
Escherichia coli
-
D-fructose + phosphate
-
Escherichia coli
-
3.1.3.10
D-glucose 1-phosphate + H2O
Escherichia coli
-
D-glucose + phosphate
-
Escherichia coli
-
3.1.3.10
D-glucose 6-phosphate + H2O
Escherichia coli
-
D-glucose + phosphate
-
Escherichia coli
-
3.1.3.10
D-ribose 5-phosphate + H2O
Escherichia coli
-
D-ribose + phosphate
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.10
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.10
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.10
4-nitrophenyl phosphate + H2O
-
134576
Escherichia coli
4-nitrophenol + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-fructose 1,6-diphosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-fructose 1-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-fructose 1-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-fructose 6-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-fructose 6-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-glucose 1-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-glucose 1-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-glucose 6-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-glucose 6-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-ribose 5-phosphate + H2O
-
134576
Escherichia coli
D-ribose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
glycerol 2-phosphate + H2O
-
134576
Escherichia coli
glycerol + phosphate
-
134576
Escherichia coli
?
3.1.3.10
glycerol 3-phosphate + H2O
-
134576
Escherichia coli
glycerol + phosphate
-
134576
Escherichia coli
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.10
dimer
1 * 44000 + 1 * 44000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.10
37
-
temperature used in the assay with 25 mM 4-nitrophenyl phosphate in 50 mM potassium phthalate-HCl buffer pH 4.5
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.10
4
5
with 4-nitrophenyl phosphate 25 mM as substrate
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.10
3
9
with 4-nitrophenyl-phosphate 25 mM as substrate
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.10
in vivo cloning technique with plasmid Mu dII4042, expression in Escherichia coli strain SBS1295
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.10
0.5
-
D-glucose 1-phosphate
-
Escherichia coli
3.1.3.10
15
-
D-glucose 6-phosphate
-
Escherichia coli
3.1.3.10
25
-
D-fructose 6-phosphate
-
Escherichia coli
3.1.3.10
30
-
4-nitrophenyl phosphate
-
Escherichia coli
3.1.3.10
30
-
D-ribose 5-phosphate
-
Escherichia coli
3.1.3.10
50
-
D-Fructose 1-phosphate
-
Escherichia coli
3.1.3.10
100
-
Glycerol 2-phosphate
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.10
periplasm
periplasm extracts made by osmotic shock of strain SBS1405(pEP1376)
Escherichia coli
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.10
44000
-
SDS-Page of osmotic shock fluid of strain SBS1405(pEP1376)
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.10
D-fructose 1,6-diphosphate + H2O
Escherichia coli
-
D-fructose + phosphate
-
Escherichia coli
-
3.1.3.10
D-fructose 1-phosphate + H2O
Escherichia coli
-
D-fructose + phosphate
-
Escherichia coli
-
3.1.3.10
D-fructose 6-phosphate + H2O
Escherichia coli
-
D-fructose + phosphate
-
Escherichia coli
-
3.1.3.10
D-glucose 1-phosphate + H2O
Escherichia coli
-
D-glucose + phosphate
-
Escherichia coli
-
3.1.3.10
D-glucose 6-phosphate + H2O
Escherichia coli
-
D-glucose + phosphate
-
Escherichia coli
-
3.1.3.10
D-ribose 5-phosphate + H2O
Escherichia coli
-
D-ribose + phosphate
-
Escherichia coli
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.10
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.10
4-nitrophenyl phosphate + H2O
-
134576
Escherichia coli
4-nitrophenol + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-fructose 1,6-diphosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-fructose 1-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-fructose 1-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-fructose 6-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-fructose 6-phosphate + H2O
-
134576
Escherichia coli
D-fructose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-glucose 1-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-glucose 1-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-glucose 6-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
-
3.1.3.10
D-glucose 6-phosphate + H2O
-
134576
Escherichia coli
D-glucose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
D-ribose 5-phosphate + H2O
-
134576
Escherichia coli
D-ribose + phosphate
-
134576
Escherichia coli
?
3.1.3.10
glycerol 2-phosphate + H2O
-
134576
Escherichia coli
glycerol + phosphate
-
134576
Escherichia coli
?
3.1.3.10
glycerol 3-phosphate + H2O
-
134576
Escherichia coli
glycerol + phosphate
-
134576
Escherichia coli
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.10
dimer
1 * 44000 + 1 * 44000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.10
37
-
temperature used in the assay with 25 mM 4-nitrophenyl phosphate in 50 mM potassium phthalate-HCl buffer pH 4.5
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.10
4
5
with 4-nitrophenyl phosphate 25 mM as substrate
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.10
3
9
with 4-nitrophenyl-phosphate 25 mM as substrate
Escherichia coli