Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pradel, E.; Boquet, P.L.
    Acid phosphatases of Escherichia coli: molecular cloning and analysis of agp, the structural gene for a periplasmic acid glucose phosphatase (1988), J. Bacteriol., 170, 4916.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.10 in vivo cloning technique with plasmid Mu dII4042, expression in Escherichia coli strain SBS1295 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.10 0.5
-
 D-glucose 1-phosphate
-
 Escherichia coli
3.1.3.10 15
-
 D-glucose 6-phosphate
-
 Escherichia coli
3.1.3.10 25
-
 D-fructose 6-phosphate
-
 Escherichia coli
3.1.3.10 30
-
 4-nitrophenyl phosphate
-
 Escherichia coli
3.1.3.10 30
-
 D-ribose 5-phosphate
-
 Escherichia coli
3.1.3.10 50
-
 D-Fructose 1-phosphate
-
 Escherichia coli
3.1.3.10 100
-
 Glycerol 2-phosphate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.10 periplasm periplasm extracts made by osmotic shock of strain SBS1405(pEP1376) Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.10 44000
-
 SDS-Page of osmotic shock fluid of strain SBS1405(pEP1376) Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.10 D-fructose 1,6-diphosphate + H2O Escherichia coli
-
 D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 1-phosphate + H2O Escherichia coli
-
 D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 6-phosphate + H2O Escherichia coli
-
 D-fructose + phosphate
-
 ?
3.1.3.10 D-glucose 1-phosphate + H2O Escherichia coli
-
 D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O Escherichia coli
-
 D-glucose + phosphate
-
 ?
3.1.3.10 D-ribose 5-phosphate + H2O Escherichia coli
-
 D-ribose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.10 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.10
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.10 4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?
3.1.3.10 D-fructose 1,6-diphosphate + H2O
-
 Escherichia coli D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 1-phosphate + H2O
-
 Escherichia coli D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 6-phosphate + H2O
-
 Escherichia coli D-fructose + phosphate
-
 ?
3.1.3.10 D-glucose 1-phosphate + H2O
-
 Escherichia coli D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O
-
 Escherichia coli D-glucose + phosphate
-
 ?
3.1.3.10 D-ribose 5-phosphate + H2O
-
 Escherichia coli D-ribose + phosphate
-
 ?
3.1.3.10 glycerol 2-phosphate + H2O
-
 Escherichia coli glycerol + phosphate
-
 ?
3.1.3.10 glycerol 3-phosphate + H2O
-
 Escherichia coli glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.10 dimer 1 * 44000 + 1 * 44000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.3.10 acid phosphatase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.10 37
-
 temperature used in the assay with 25 mM 4-nitrophenyl phosphate in 50 mM potassium phthalate-HCl buffer pH 4.5 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.10 4 5 with 4-nitrophenyl phosphate 25 mM as substrate Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.10 3 9 with 4-nitrophenyl-phosphate 25 mM as substrate Escherichia coli