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Literature summary extracted from

  • Gurucharan Reddy, L.; Shankar, V.
    Preparation and properties of RNase T2 immobilized on concanavalin A-sepharose (1989), Appl. Biochem. Biotechnol., 22, 237-246.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.19 additional information EDTA activates soluble enzyme, no effect on immobilized Aspergillus oryzae

General Stability

EC Number General Stability Organism
4.6.1.19 stability of immobilized enzyme Aspergillus oryzae
4.6.1.19 urea, 8 M, soluble and immobilized enzyme, stable Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.19 Hg2+
-
 Aspergillus oryzae
4.6.1.19 Zn2+
-
 Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.19 additional information
-
 additional information RNA: 555 mg/ml soluble enzyme, 266 mg/ml immobilized enzyme Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.19 CuSO4 activates immobilized enzyme, inhibits soluble Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.19 Aspergillus oryzae
-
 immmobilized enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.19 partial Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.19 commercial preparation Takadiastase powder Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.19 additional information
-
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.19 RNA + H2O
-
 Aspergillus oryzae 3'-phosphomononucleotides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.19 62
-
 soluble and immobilized enzyme Aspergillus oryzae
4.6.1.19 65
-
-
 Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.6.1.19 40 70 40°C: about 60% of activity maximum, soluble and immobilized enzyme, 70°C: about 70% immobilized enzyme, 40% soluble enzyme, of activity maximum Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.19 50
-
 pH 4.5, 1 h, soluble enzyme: about 29% loss of activity, immobilized enzyme: about 10% loss of activity Aspergillus oryzae
4.6.1.19 70
-
 pH 4.5, 1 h, soluble form: complete loss of activity, immobilized form: about 40% loss of activity Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.19 4.5
-
 soluble and immobilized enzyme Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.6.1.19 3.5 5.5 pH 3.5, 5.5: about 70% of activity maximum, soluble and immobilized enzyme Aspergillus oryzae
4.6.1.19 3.5 6 pH 3.5: 80% of activity maximum, soluble and immobilized form, pH 6: soluble form 100%, immobilized form 90% of maximum acitivity Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.6.1.19 3.5
-
 1 h, about 20% loss of activity, soluble and immobilized form Aspergillus oryzae
4.6.1.19 4.5 5.5 1 h Aspergillus oryzae
4.6.1.19 6
-
 1 h, about 10% loss of activity Aspergillus oryzae