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Literature summary extracted from

  • Bernarde, G.
    Spleen acid deoxyribonuclease (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 271-287.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.22.1 cysteine protects the enzyme against traces of heavy metals Sus scrofa
3.1.22.1 EDTA activator Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.22.1 actinomycin D strong inhibition, coupling to the substrate not to the enzyme Sus scrofa
3.1.22.1 DNA inhibitory above 0.4 mg/ml Sus scrofa
3.1.22.1 HPO42- slightly inhibitory above pH 5.0 Sus scrofa
3.1.22.1 iodoacetamide slight inhibitory Sus scrofa
3.1.22.1 iodoacetate strongly inhibitory Sus scrofa
3.1.22.1 Mg2+ slightly inhibitory at 10 mM and pH 4.5, inhibitory effects depending on enzyme purity Sus scrofa
3.1.22.1 N-bromosuccinimide strong inhibitory Sus scrofa
3.1.22.1 Protein inhibitor inhibition below pH 5.6, forming a DNase-inhibitor complex Sus scrofa
3.1.22.1 rRNA specific competitive inhibition Sus scrofa
3.1.22.1 SO42- very strong inhibition, particulary above pH 4.5 Sus scrofa
3.1.22.1 succinate buffer activity less than 3% of that in acetate buffer at pH 5.0 Sus scrofa
3.1.22.1 tRNA specific competitive inhibition Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.22.1 lysosome
-
Sus scrofa 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.22.1 38000
-
-
Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.22.1 DNA + H2O Sus scrofa involvement in recombination, replication and degradation of DNA oligonucleotides + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.22.1 glycoprotein glucosamine and neutral sugar residues Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.22.1 near homogeneity by chromatography steps Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.22.1 spleen multiple isoforms Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.22.1 bis(p-nitrophenyl) phosphate + H2O slow hydrolysis at optimal pH from 5.6 to 5.9 Sus scrofa p-nitrophenol + p-nitrophenyl phosphate:
-
?
3.1.22.1 deoxyribonucleoside 5'-phosphates p-nitrophenyl esters + H2O
-
Sus scrofa p-nitrophenol + deoxyribonucleoside 5'-phosphates
-
?
3.1.22.1 DNA + H2O double-strand and single-strand scission Sus scrofa 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides formation of oligonucleotides of an average chain length from 14 to 100 in the middle activity phase, purine nucleotides form about 75% of the 3'-terminals, formation of oligonucleotides of an average chain length from 6 to 14 in the terminal activity phase ?
3.1.22.1 DNA + H2O involvement in recombination, replication and degradation of DNA Sus scrofa oligonucleotides + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.22.1 dimer
-
Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.22.1 4.8
-
at low ionic strength DNase is active at neutral pH Sus scrofa