EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.22.1 | cysteine | protects the enzyme against traces of heavy metals | Sus scrofa | |
3.1.22.1 | EDTA | activator | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.22.1 | actinomycin D | strong inhibition, coupling to the substrate not to the enzyme | Sus scrofa | |
3.1.22.1 | DNA | inhibitory above 0.4 mg/ml | Sus scrofa | |
3.1.22.1 | HPO42- | slightly inhibitory above pH 5.0 | Sus scrofa | |
3.1.22.1 | iodoacetamide | slight inhibitory | Sus scrofa | |
3.1.22.1 | iodoacetate | strongly inhibitory | Sus scrofa | |
3.1.22.1 | Mg2+ | slightly inhibitory at 10 mM and pH 4.5, inhibitory effects depending on enzyme purity | Sus scrofa | |
3.1.22.1 | N-bromosuccinimide | strong inhibitory | Sus scrofa | |
3.1.22.1 | Protein inhibitor | inhibition below pH 5.6, forming a DNase-inhibitor complex | Sus scrofa | |
3.1.22.1 | rRNA | specific competitive inhibition | Sus scrofa | |
3.1.22.1 | SO42- | very strong inhibition, particulary above pH 4.5 | Sus scrofa | |
3.1.22.1 | succinate buffer | activity less than 3% of that in acetate buffer at pH 5.0 | Sus scrofa | |
3.1.22.1 | tRNA | specific competitive inhibition | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.22.1 | lysosome | - |
Sus scrofa | 5764 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.22.1 | 38000 | - |
- |
Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.22.1 | DNA + H2O | Sus scrofa | involvement in recombination, replication and degradation of DNA | oligonucleotides + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.22.1 | Sus scrofa | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.22.1 | glycoprotein | glucosamine and neutral sugar residues | Sus scrofa |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.22.1 | near homogeneity by chromatography steps | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.22.1 | spleen | multiple isoforms | Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.22.1 | bis(p-nitrophenyl) phosphate + H2O | slow hydrolysis at optimal pH from 5.6 to 5.9 | Sus scrofa | p-nitrophenol + p-nitrophenyl phosphate: | - |
? | |
3.1.22.1 | deoxyribonucleoside 5'-phosphates p-nitrophenyl esters + H2O | - |
Sus scrofa | p-nitrophenol + deoxyribonucleoside 5'-phosphates | - |
? | |
3.1.22.1 | DNA + H2O | double-strand and single-strand scission | Sus scrofa | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | formation of oligonucleotides of an average chain length from 14 to 100 in the middle activity phase, purine nucleotides form about 75% of the 3'-terminals, formation of oligonucleotides of an average chain length from 6 to 14 in the terminal activity phase | ? | |
3.1.22.1 | DNA + H2O | involvement in recombination, replication and degradation of DNA | Sus scrofa | oligonucleotides + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.22.1 | dimer | - |
Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.22.1 | 4.8 | - |
at low ionic strength DNase is active at neutral pH | Sus scrofa |