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Literature summary extracted from
- Structural specificities of five commonly used DNA nucleases (1984), J. Mol. Biol., 176, 535-557.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.22.1 | 40000 | - |
- |
Mammalia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.22.1 | DNA + H2O | Mammalia | prefers to cut purine-rich steps in duplex-DNA, will also attack heat-denatured and single-stranded substrates | oligonucleotides + ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.22.1 | Mammalia | - |
commercial enzyme | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.22.1 | DNA + H2O | prefers to cut purine-rich steps in duplex-DNA, will also attack heat-denatured and single-stranded substrates | Mammalia | oligonucleotides + ? | - |
? | |
| 3.1.22.1 | duplex-oligonucleotide + H2O | 20- and 16mers with specific sequences and structures like hairpin and duplex DNA, can cut bonds in a loop at nearly the same rate as in duplex DNA, doesn't require phosphate exposure or a double-stranded track | Mammalia | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.22.1 | 4.8 | - |
- |
Mammalia |
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