Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Endlich, B.; Linn, S.
    Type I restriction enzymes (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 137-156.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.3 S-adenosyl-L-methionine required Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.3 Mg2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.3 52000
-
 alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli
3.1.21.3 55000
-
 alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
3.1.21.3 60000
-
 alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
3.1.21.3 62000
-
 alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli
3.1.21.3 135000
-
 alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli
3.1.21.3 135000
-
 alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
3.1.21.3 400000
-
 EcoK, sucrose density gradient sedimentation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.3 additional information Salmonella enterica subsp. enterica serovar Typhimurium enzyme restricts the exchange of genetic material between bacteria of different strains or species ?
-
 ?
3.1.21.3 additional information Escherichia coli enzyme restricts the exchange of genetic material between bacteria of different strains or species ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.3 Escherichia coli
-
 enzyme EcoR
-
3.1.21.3 Escherichia coli
-
 enzyme EcoK
-
3.1.21.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
 enzyme SA and SB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.3 enzyme EcoB Escherichia coli
3.1.21.3 enzyme EcoK Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.3 duplex DNA + ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?
3.1.21.3 duplex DNA + ATP the site of restriction cleavage is random, occuring between 1 and 5 kb from the recognition site. The modification methylase acts directly at the recognition sequence Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?
3.1.21.3 additional information enzyme restricts the exchange of genetic material between bacteria of different strains or species Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.1.21.3 additional information enzyme restricts the exchange of genetic material between bacteria of different strains or species Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.3 octamer alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
3.1.21.3 pentamer alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.21.3 EcoK
-
 Escherichia coli
3.1.21.3 EcoR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.21.3 167
-
 ATP at 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.3 8
-
 nuclease reaction Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.21.3 ATP required Escherichia coli
3.1.21.3 ATP is degraded during reaction Escherichia coli