EC Number | General Stability | Organism |
---|---|---|
3.1.21.1 | Ca2+ is able to protect DNase completely against inactivation by trypsin | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | 2-mercaptoethanol | inactivation, reversal by the addition of 4 mM CaCl2, no inactivation if CaCl2 is present during the reducing reaction | Bos taurus | |
3.1.21.1 | calf spleen inhibitor protein II | molecular weight: 59000 Da, forms an inhibitory uni-uni molecular complex with DNase I, maximum stability at pH 7 | Bos taurus | |
3.1.21.1 | calf thymus inhibitor protein | molecular weight: 49000 Da, maximum stability at pH 6 | Bos taurus | |
3.1.21.1 | iodoacetate | formation of a 3-carboxymethyl histidine per molecule, in the presence of 0.1 M Mn2+ gradual inactivation | Bos taurus | |
3.1.21.1 | NaCl | - |
Bos taurus | |
3.1.21.1 | oligonucleotides | competitive inhibition | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | Ca2+ | no lag phase in the reaction obtained, if only Ca2+ is present or both Mg2+ and Ca2+ are present, double strand digestion preferred | Bos taurus | |
3.1.21.1 | Co2+ | reaction performs mainly double strand scissions, Co2+ is a better activator than other divalent metals | Bos taurus | |
3.1.21.1 | Mg2+ | only single strand cleavage occurs during the lag period of the reaction if only Mg2+ is present | Bos taurus | |
3.1.21.1 | Mn2+ | reaction performs mainly double strand scissions | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 31000 | - |
amino acid analysis | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | double-stranded DNA + H2O | Bos taurus | preference for double-stranded DNA | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? | |
3.1.21.1 | double-stranded DNA + H2O | Bos taurus | major function is to inflict nicks during early stages of hydrolytic attack on DNA, participation in repair phenomena | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.1 | Bos taurus | - |
4 active compounds: A, B, C, D | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.21.1 | glycoprotein | DNase A: 2 N-acetylglucosamine residues, 6 mannose residues, DNase B: 1 sialic acid residue, 3 N-acetylglucosamine residues, 5 mannose residues | Bos taurus |
3.1.21.1 | glycoprotein | DNase A with a neutral carbohydrate side-chain, DNase B with a sialic acid carbohydrate side-chain | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.21.1 | pancreas | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | d-ApApTp + H2O | - |
Bos taurus | pTp + d-ApA | - |
? | |
3.1.21.1 | DNA + H2O | preference for native DNA | Bos taurus | 5'-phosphooligonucleotides + ? | produces nicks on one strand in preference to scission of both strands, autoretardation causes the continuous formation of products which are poorer substrates than those from which they are derived | ? | |
3.1.21.1 | double-stranded DNA + H2O | preference for double-stranded DNA | Bos taurus | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? | |
3.1.21.1 | double-stranded DNA + H2O | major function is to inflict nicks during early stages of hydrolytic attack on DNA, participation in repair phenomena | Bos taurus | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.1 | DNase A | - |
Bos taurus |
3.1.21.1 | DNase B | - |
Bos taurus |
3.1.21.1 | DNase C | - |
Bos taurus |
3.1.21.1 | DNase D | - |
Bos taurus |