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Literature summary extracted from

  • Nagae, S.; Nakayama, J.; Nakano, I.; Anai, M.
    Purification and properties of a neutral endodeoxyribonuclease from rat small intestinal mucosa (1982), Biochemistry, 21, 1339-1344.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.1 bovine serum albumin 0.01 mg/reaction volume stimulates about 50% at both pH 6.2 and 7.7 Rattus sp.
3.1.21.1 N-ethylmaleimide stimulation about 10-20% at both pH 6.2 and 7.7 Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 2-mercaptoethanol inhibition, but reversal by addition of 3 mM CaCl2 Rattus sp.
3.1.21.1 Cu2+ complete inhibition Rattus sp.
3.1.21.1 EDTA
-
 Rattus sp.
3.1.21.1 G-actin
-
 Rattus sp.
3.1.21.1 iodoacetate complete inhibition Rattus sp.
3.1.21.1 NaCl
-
 Rattus sp.
3.1.21.1 Zn2+ complete inhibition Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.1 extracellular
-
 Rattus sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+
-
 Rattus sp.
3.1.21.1 Mg2+ activation synergistic with Ca2+ Rattus sp.
3.1.21.1 Mn2+
-
 Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 32000
-
 gel filtration, sucrose density gradient centrifugation Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Rattus sp. introduces preferentially breaks on the 5' side of dT 5'-phosphooligonucleotides + ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.21.1 Ethylene glycol 80% loss of activity during overnight at 0°C Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 to homogeneity by affinity chromatography Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 intestinal mucosa
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 14.2
-
-
 Rattus sp.

Storage Stability

EC Number Storage Stability Organism
3.1.21.1 -20°C, 20 mM Tris-HCl, pH 7.5, 30% glycerol, 6 months Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O degrades native DNA 1.5times faster than denatured DNA Rattus sp. 5'-phosphooligonucleotides + ? half the 5'-termini are deoxythymidinemonophosphate, 90% of the products are larger than pentanucleotides ?
3.1.21.1 double-stranded DNA + H2O introduces preferentially breaks on the 5' side of dT Rattus sp. 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 heat-denatured DNA + H2O
-
 Rattus sp. 5'-phosphooligonucleotides + ? 90% of the products are larger than pentanucleotides ?
3.1.21.1 relaxed circular plasmid DNA + H2O acts more rapidly on circular duplex DNA than on supercoiled DNA Rattus sp. 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 supercoiled plasmid DNA + H2O
-
 Rattus sp. linear DNA + ? superhelical form converted to circular-relaxed and linear forms ?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 monomer
-
 Rattus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 -30
-
 no loss of activity in crude extract Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 6.2
-
 in 25 mM cacodylate hydrochlorid and 3 mM Co2+ Rattus sp.
3.1.21.1 7.7
-
 in 25 mM Tris-HCl and 1 mM Mn2+ Rattus sp.