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Literature summary extracted from

  • Murata, K.; Inoue, Y.; Watanabe, K.; Fukuda, Y.; Saikusa, T.; Shimosaka, M.; Kimura, A.
    Metabolism of alpha-ketoaldehydes in yeast: purification and characterization of glyoxalase II from Saccharomyces cerevisiae (1986), Agric. Biol. Chem., 50, 135-142.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 coenzyme A
-
 Saccharomyces cerevisiae
3.1.2.6 DTT 1.25 mM, 60% inhibition Saccharomyces cerevisiae
3.1.2.6 glutathione 8.0 mM, 43% loss of activity Saccharomyces cerevisiae
3.1.2.6 Hg2+ HgCl2, 0.5 mM, complete inhibition Saccharomyces cerevisiae
3.1.2.6 iodoacetate
-
 Saccharomyces cerevisiae
3.1.2.6 PCMB 0.3 mM, complete inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.0007
-
 S-Lactoylglutathione
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.6 19000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 1.34
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 lactoylglutathione + H2O
-
 Saccharomyces cerevisiae D-lactate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.6 monomer 1 * 19000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 45
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.6 40
-
 pH 7.0, 14 min, 50% loss of activity Saccharomyces cerevisiae
3.1.2.6 50
-
 pH 7.0, 2 min, 50% loss of activity Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.6 3
-
 stable in 0.001 N HCl at 40°C for 15 min Saccharomyces cerevisiae
3.1.2.6 9
-
 rapid loss of activity above Saccharomyces cerevisiae