Any feedback?
Literature summary extracted from
- Phospholipase A2 from bee venom (1981), Methods Enzymol., 71, 698-702.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Acylating agents | example: fatty acid imidazolides | Apis mellifera | |
| 3.1.1.4 | EDTA | in small quantities activating: one-tenth the Ca2+-concentration | Apis mellifera |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | EDTA | - |
Apis mellifera | |
| 3.1.1.4 | Maleic anhydride | - |
Apis mellifera | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Ba2+ | can replace Ca2+ but enzyme shows decreased activity | Apis mellifera | |
| 3.1.1.4 | Ca2+ | - |
Apis mellifera | |
| 3.1.1.4 | Mg2+ | can replace Ca2+, decreased activity | Apis mellifera | |
| 3.1.1.4 | Sr2+ | can replace Ca2+, decreased activity | Apis mellifera | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | 1,2-diacyl-sn-glycero-3-phosphatide + H2O | Apis mellifera | - |
? | - |
? | |
| 3.1.1.4 | phospholipids + H2O | Apis mellifera | allergenic | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Apis mellifera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | dialysis, column chromatography | Apis mellifera |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | venom | - |
Apis mellifera | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | 1,2-diacyl-sn-glycero-3-phosphatide + H2O | - |
Apis mellifera | 1-acyl-sn-glycero-3-phosphatide + fatty acid | - |
? | |
| 3.1.1.4 | 1,2-diacyl-sn-glycero-3-phosphatide + H2O | - |
Apis mellifera | ? | - |
? | |
| 3.1.1.4 | 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O | - |
Apis mellifera | 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate | - |
? | |
| 3.1.1.4 | 1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O | - |
Apis mellifera | 1-lauroyl-sn-glycero-3-phosphocholine + laureate | - |
? | |
| 3.1.1.4 | 1,2-dimyristoyl-sn-phosphatidylcholine + H2O | - |
Apis mellifera | 1-myristoyl-phosphorylcholine + myristic acid | - |
? | |
| 3.1.1.4 | 1,2-dipalmitoyl-phosphatidylcholine + H2O | - |
Apis mellifera | 1-palmitoyl-glycerophosphorylcholine + palmitic acid | - |
? | |
| 3.1.1.4 | 1-oleoyl-2-isolauroyl phosphatidylethanolamine + H2O | - |
Apis mellifera | 1-oleoyl phosphatidylethanolamine + isolauric acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Apis mellifera | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylglycerol + H2O | - |
Apis mellifera | 1-acylglycerol + fatty acid | - |
? | |
| 3.1.1.4 | phospholipids + H2O | allergenic | Apis mellifera | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | dimer | dimerization occurs in concentrated aqueous solution, no data concerning MW | Apis mellifera |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 20 | - |
lyophilized venom PLA2, extremely stable | Apis mellifera |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 8 | - |
substrate: 1,2-dihexanoyl-sn-glycero-3-phosphocholine, in 75% propanol and 25% water | Apis mellifera |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
