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Literature summary extracted from

  • Deems, R.A.; Dennis, E.A.
    Phospholipase A2 from cobra venom (Naja naja naja) (1981), Methods Enzymol., 71, 703-710.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 phosphatidylcholine activates hydrolysis of phosphatidylethanolamine Naja naja
3.1.1.4 sphingomyelin activates hydrolysis of phosphatidylethanolamine Naja naja

General Stability

EC Number General Stability Organism
3.1.1.4 guanidine-HCL, PLA2 retains 60% of activity when assay contains 6 M of guanidine-HCl Naja naja

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 Ba2+
-
 Naja naja
3.1.1.4 p-bromophenacyl bromide
-
 Naja naja
3.1.1.4 Sr2+ competitive inhibition Naja naja

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 2 5 phosphatidylcholine Triton X-100/phospholipid: 2/1 Naja naja

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Naja naja

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 13000
-
 gel filtration, SDS-PAGE, analytical ultracentrifugation Naja naja

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O Naja naja
-
 lysophosphatidylcholine + a fatty acid
-
 ?
3.1.1.4 phospholipids + H2O Naja naja
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.4 Acetone PLA2 regains almost full activity when added to assay mixture Naja naja
3.1.1.4 benzene PLA2 regains almost full activity when added to assay mixture Naja naja
3.1.1.4 chloroform PLA2 regains almost full activity when added to assay mixture Naja naja
3.1.1.4 ether PLA2 regains almost full activity when added to assay mixture Naja naja

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Naja naja
-
 cobra
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 column chromatography, 15fold Naja naja

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 1129
-
 substrate: 1,2-diacyl-phosphatidylcholine Naja naja
3.1.1.4 2000
-
 Triton X-100/phospholipid: 2/1 Naja naja

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 frozen, PLA2 concentration below 2 mg/ml, no loss of activity or precipitation for up to 12 months Naja naja

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-diacyl-sn-glycero-3-phosphorylcholine + H2O
-
 Naja naja 1-acyl-sn-glycero-3-phosphorylcholine + fatty acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Naja naja lysophosphatidylcholine + a fatty acid
-
 ?
3.1.1.4 phosphatidylethanolamine + H2O
-
 Naja naja 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
3.1.1.4 phosphatidylserine + H2O
-
 Naja naja 1-acylglycerophosphoserine + fatty acid
-
 ?
3.1.1.4 phospholipids + H2O
-
 Naja naja ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 dimer 2 * 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, dimerization occurs at enzyme concentrations between 0.1 and 2 mg PLA2 per ml Naja naja
3.1.1.4 monomer monomeric when enzyme concentration is below 0.05 mg PLA2 per ml, 13000, gel filtration, SDS-PAGE, analytical ultracentifugation Naja naja
3.1.1.4 polymer 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, polimerization at enzyme concentrations above 5mg PLA2 per ml Naja naja

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 100
-
 for 10 min at pH 3-4, only 5% loss in activity Naja naja

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.4 7 9 substrate: 1,2-diacyl-phosphatidylcholine Naja naja