EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | phosphatidylcholine | activates hydrolysis of phosphatidylethanolamine | Naja naja | |
3.1.1.4 | sphingomyelin | activates hydrolysis of phosphatidylethanolamine | Naja naja |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.4 | guanidine-HCL, PLA2 retains 60% of activity when assay contains 6 M of guanidine-HCl | Naja naja |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | Ba2+ | - |
Naja naja | |
3.1.1.4 | p-bromophenacyl bromide | - |
Naja naja | |
3.1.1.4 | Sr2+ | competitive inhibition | Naja naja |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.4 | 2 | 5 | phosphatidylcholine | Triton X-100/phospholipid: 2/1 | Naja naja |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | Ca2+ | - |
Naja naja |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 13000 | - |
gel filtration, SDS-PAGE, analytical ultracentrifugation | Naja naja |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | phosphatidylcholine + H2O | Naja naja | - |
lysophosphatidylcholine + a fatty acid | - |
? | |
3.1.1.4 | phospholipids + H2O | Naja naja | - |
? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.4 | Acetone | PLA2 regains almost full activity when added to assay mixture | Naja naja |
3.1.1.4 | benzene | PLA2 regains almost full activity when added to assay mixture | Naja naja |
3.1.1.4 | chloroform | PLA2 regains almost full activity when added to assay mixture | Naja naja |
3.1.1.4 | ether | PLA2 regains almost full activity when added to assay mixture | Naja naja |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.4 | Naja naja | - |
cobra | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.4 | column chromatography, 15fold | Naja naja |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 1129 | - |
substrate: 1,2-diacyl-phosphatidylcholine | Naja naja |
3.1.1.4 | 2000 | - |
Triton X-100/phospholipid: 2/1 | Naja naja |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.4 | frozen, PLA2 concentration below 2 mg/ml, no loss of activity or precipitation for up to 12 months | Naja naja |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | 1,2-diacyl-sn-glycero-3-phosphorylcholine + H2O | - |
Naja naja | 1-acyl-sn-glycero-3-phosphorylcholine + fatty acid | - |
? | |
3.1.1.4 | phosphatidylcholine + H2O | - |
Naja naja | lysophosphatidylcholine + a fatty acid | - |
? | |
3.1.1.4 | phosphatidylethanolamine + H2O | - |
Naja naja | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
3.1.1.4 | phosphatidylserine + H2O | - |
Naja naja | 1-acylglycerophosphoserine + fatty acid | - |
? | |
3.1.1.4 | phospholipids + H2O | - |
Naja naja | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.4 | dimer | 2 * 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, dimerization occurs at enzyme concentrations between 0.1 and 2 mg PLA2 per ml | Naja naja |
3.1.1.4 | monomer | monomeric when enzyme concentration is below 0.05 mg PLA2 per ml, 13000, gel filtration, SDS-PAGE, analytical ultracentifugation | Naja naja |
3.1.1.4 | polymer | 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, polimerization at enzyme concentrations above 5mg PLA2 per ml | Naja naja |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 100 | - |
for 10 min at pH 3-4, only 5% loss in activity | Naja naja |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 7 | 9 | substrate: 1,2-diacyl-phosphatidylcholine | Naja naja |