Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, R.; Dodia, C.R.; Jain, M.K.; Fisher, A.B.
    Purification and characterization of a calcium-independent acidic phospholipase A2 from rat lung (1994), Biochem. J., 304, 131-137.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol MJ33, competitive inhibitor Rattus norvegicus
3.1.1.4 Ba2+
-
 Rattus norvegicus
3.1.1.4 Ca2+ 30% decrease of activity at 5-10 mM Rattus norvegicus
3.1.1.4 Mg2+
-
 Rattus norvegicus
3.1.1.4 surfactant protein A
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 lamellar body
-
 Rattus norvegicus 42599
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 15000
-
 gel filtration, gel electrophoresis, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 560fold Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 lung
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.04
-
 renatured enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine Rattus norvegicus
3.1.1.4 0.56
-
 native enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -20°C, activity increased 3fold after storage for one week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Rattus norvegicus 1-acylglycerophosphocholine + fatty acid
-
 ?
3.1.1.4 phospholipids + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 4
-
-
 Rattus norvegicus