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Literature summary extracted from

  • Mori, S.; Nakata,Y.; Endo, H.
    Enzymatic properties of polyethylene glycol-modified cholesterol esterase in organic solvents (1992), Biotechnol. Appl. Biochem., 16, 101-105.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Pseudomonas sp. long-chain fatty acid cholesterol esters more easily synthesized, synthetic activity highest in chloroform, activity enhanced in degree of unsaturation of fatty acids cholesterol + fatty acid
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 r

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.13 chloroform most suitable for cholesteryl linoleate synthesis in polyethylene glycol-modified enzyme Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Pseudomonas sp.
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O long-chain fatty acid cholesterol esters more easily synthesized, synthetic activity highest in chloroform, activity enhanced in degree of unsaturation of fatty acids Pseudomonas sp. cholesterol + fatty acid
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 r
3.1.1.13 cholesteryl laurate + H2O
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 Pseudomonas sp. cholesterol + lauric acid
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 r
3.1.1.13 cholesteryl linoleate + H2O synthesis of cholesterol linoleate from polyethylene glycol-modified enzyme only in water-immiscible solvents Pseudomonas sp. cholesterol + linoleic acid
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 r
3.1.1.13 cholesteryl myristate + H2O
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 Pseudomonas sp. cholesterol + myristic acid
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 r
3.1.1.13 cholesteryl oleate + H2O
-
 Pseudomonas sp. cholesterol + oleic acid
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 r
3.1.1.13 cholesteryl palmitate + H2O
-
 Pseudomonas sp. cholesterol + palmitic acid
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 r
3.1.1.13 cholesteryl stearate + H2O
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 Pseudomonas sp. cholesterol + stearic acid
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 r