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Literature summary extracted from

  • Walsh, P.J.
    Purification and characterization of two allozymic forms of octopine dehydrogenase from california populations of Metridium senile (1981), J. Comp. Physiol., 143, 213-222.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.11 3-hydroxypyruvate inhibitor of octopine formation Metridium senile
1.5.1.11 glyoxylate inhibitor of octopine formation Metridium senile
1.5.1.11 homoarginine inhibitor of octopine formation Metridium senile
1.5.1.11 octopine product inhibition Metridium senile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.11 additional information
-
 additional information
-
 Metridium senile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.11 34900
-
 1 * 34900, SDS-PAGE Metridium senile
1.5.1.11 40000
-
 gel filtration Metridium senile

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.11 Metridium senile
-
 2 allozymic forms are distributed in a highly population-specific manner, enzyme variants: ss, ff, and sf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.11
-
 Metridium senile

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.11 45.5
-
-
 Metridium senile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.11 Canavanine + pyruvate + NADH 57.6-92.6% of the activity with Arg, depending on enzyme variant Metridium senile N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
 ?
1.5.1.11 Homoarginine + pyruvate + NADH 58.6-70.8% of activity with Arg, depending on enzyme variant Metridium senile N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
 ?
1.5.1.11 L-Arg + 2-oxobutanoate + NADH 20.6-29.0% of activity with Arg Metridium senile N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
 ?
1.5.1.11 L-Arg + oxaloacetate + NADH at 6.8-14.4% of activity with L-Arg Metridium senile ?
-
 ?
1.5.1.11 L-Arg + pyruvate + NADH
-
 Metridium senile N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?
1.5.1.11 L-Lys + pyruvate + NADH 87.2-92.6% of activity with Arg, depending on enzyme variant Metridium senile Lysopine + NAD+ + H2O
-
 ?
1.5.1.11 additional information no activity with 2-hydroxybutanoate Metridium senile ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.11 monomer 1 * 34900, SDS-PAGE Metridium senile

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.11 NADH
-
 Metridium senile