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Literature summary extracted from

  • Huennekens, F.M.; Henderson, G.B.; Vitols, K.S.; Grimshaw, C.E.
    Enzymatic activation of 5-formyltetrahydrofolate via conversion to 5,10-methenyltetrahydrofolate (1984), Adv. Enzyme Regul., 22, 3-13.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.3.2 enzyme loses activity when subjected to dialysis or concentration by ultrafiltration Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.0006
-
5-formyltetrahydrofolate
-
Lacticaseibacillus casei
6.3.3.2 0.001
-
ATP
-
Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.3.2 Mg2+ required Oryctolagus cuniculus
6.3.3.2 Mg2+ required Ovis aries
6.3.3.2 Mg2+ required Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.2 23000
-
gel filtration Lacticaseibacillus casei
6.3.3.2 25000
-
gel filtration Oryctolagus cuniculus
6.3.3.2 26500 29000 gel filtration, HPLC gel filtration, equilibrium ultracentrifugation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Gallus gallus
-
-
-
6.3.3.2 Lacticaseibacillus casei
-
-
-
6.3.3.2 Mus musculus
-
-
-
6.3.3.2 Oryctolagus cuniculus
-
-
-
6.3.3.2 Ovis aries
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
6.3.3.2 rapid loss of activity unless kept in the presence of reducing agents, e.g. DTT Lacticaseibacillus casei

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2
-
Lacticaseibacillus casei

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.3.2 ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate mechanism is proposed to involve phosphorylation of the formyl group to create an enol phosphate Lacticaseibacillus casei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.3.2 colonic cancer cell line
-
Mus musculus
-
6.3.3.2 L-1210 cell L210 mouse leukemia cells Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.2 3.8
-
-
Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
Gallus gallus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
Mus musculus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
Oryctolagus cuniculus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
Ovis aries ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
Lacticaseibacillus casei ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
6.3.3.2 ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Lacticaseibacillus casei ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?

Subunits

EC Number Subunits Comment Organism
6.3.3.2 monomer 1 * 25000, SDS-PAGE Oryctolagus cuniculus
6.3.3.2 monomer 1 * 23000, SDS-PAGE Lacticaseibacillus casei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.2 additional information
-
additional information
-
Oryctolagus cuniculus
6.3.3.2 additional information
-
additional information
-
Lacticaseibacillus casei