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Literature summary extracted from

  • Meziane-Cherif, D.; Badet-Denisot, M.A.; Evers, S.; Courvalin, P.; Badet, B.
    Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583 (1994), FEBS Lett., 354, 140-142.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.2.1 epxression in Escherichia coli Enterococcus faecalis
6.3.2.4 Van B ligase is overproduced in Escherichia coli HB101 harboring recombinant plasmid pAT204 Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.2.1 1.2
-
 D-Ala cosubstrate D-Ala, binding of N-terminal Ala residue. pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 3
-
 D-2-hydroxybutanoate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 8.3
-
 D-2-hydroxyvalerate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 11.4
-
 D-lactate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 34
-
 D-Ala cosubstrate D-Ala, binding of C-terminal Ala residue. pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.3.2.4 1.2
-
 D-Ala D-Ala, N-terminal Ala, + D-Ala, VanB Enterococcus faecalis
6.3.2.4 3
-
 2-hydroxybutanoate
-
 Enterococcus faecalis
6.3.2.4 8.3
-
 2-hydroxypentanoate
-
 Enterococcus faecalis
6.3.2.4 11.4
-
 D-lactate
-
 Enterococcus faecalis
6.3.2.4 34
-
 D-Ala C-terminal Ala, reaction with D-Ala + ATP, VanB Enterococcus faecalis
6.3.2.4 69
-
 ATP VanB Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.2.1 38000
-
 x * 38000, SDS-PAGE Enterococcus faecalis
6.3.2.4 38000
-
 x * 38000, SDS-PAGE Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
6.1.2.1 Enterococcus faecalis
-
 V583, isoform VanB
-
6.3.2.4 Bacteria
-
-
-
6.3.2.4 Enterococcus faecalis
-
 V583, VanB ligase with type B vancomycin resistance
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.2.1 D-Ala + D-2-hydroxybutanoate + ATP
-
 Enterococcus faecalis D-Ala-D-2-hydroxybutanoate + ADP + phosphate
-
 ?
6.1.2.1 D-Ala + D-2-hydroxyvalerate + ATP
-
 Enterococcus faecalis D-Ala-D-2-hydroxyvalerate + ADP + phosphate
-
 ?
6.1.2.1 D-Ala + D-Ala + ATP
-
 Enterococcus faecalis D-Ala-D-Ala + ADP + phosphate
-
 ?
6.1.2.1 D-Ala + D-lactate + ATP
-
 Enterococcus faecalis D-Ala-D-lactate + ADP + phosphate
-
 ?
6.3.2.4 ATP + D-Ala + 2-hydroxybutanoate
-
 Enterococcus faecalis ADP + phosphate + D-Ala-D-2-hydroxybutanoate
-
 ?
6.3.2.4 ATP + D-Ala + 2-hydroxypentanoate
-
 Enterococcus faecalis ADP + phosphate + D-Ala-D-2-hydroxypentanoate
-
 ?
6.3.2.4 ATP + D-Ala + D-Ala
-
 Bacteria ADP + phosphate + D-Ala-D-Ala
-
 ?
6.3.2.4 ATP + D-Ala + D-Ala
-
 Enterococcus faecalis ADP + phosphate + D-Ala-D-Ala
-
 ?
6.3.2.4 ATP + D-Ala + D-lactate
-
 Enterococcus faecalis ADP + phosphate + D-Ala-D-lactate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.2.1 ? x * 38000, SDS-PAGE Enterococcus faecalis
6.3.2.4 ? x * 38000, SDS-PAGE Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.2.1 0.25
-
 D-2-hydroxybutanoate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 0.42
-
 D-2-hydroxyvalerate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 0.47
-
 D-lactate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 4.1
-
 D-Ala pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.3.2.4 additional information
-
 additional information
-
 Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.2.1 0.0414
-
 D-lactate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 0.05
-
 D-2-hydroxyvalerate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 0.084
-
 D-2-hydroxybutanoate pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis
6.1.2.1 0.12
-
 D-Ala pH not specified in the publication, temperature not specified in the publication Enterococcus faecalis