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Literature summary extracted from

  • Fischer, D.; Ebenau-Jehle, C.; Grisebach, H.
    Phytoalexin synthesis in soybean: purification and characterization of NADPH: 2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell culture (1990), Arch. Biochem. Biophys., 276, 390-395.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.51 0.05
-
 2'-Hydroxydaidzein
-
 Glycine max
1.3.1.51 0.056
-
 NADPH
-
 Glycine max
1.3.1.51 0.06
-
 2'-Hydroxyformomonetin
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.51 34700
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.51 additional information Glycine max specific role in the biosynthesis of glyceollin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.51 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.51
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.51 cell culture elicitor-induced Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.51 additional information
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.51 2'-Hydroxydaidzein + NADH 8% of the reaction with NADP+ Glycine max 2'-Hydroxydihydrodaidzein + NAD+
-
 ?
1.3.1.51 2'-Hydroxydaidzein + NADPH
-
 Glycine max 2'-Hydroxydihydrodaidzein + NADP+
-
 ?
1.3.1.51 2'-Hydroxyformomonetin + NADPH
-
 Glycine max 2'-Hydroxydihydroformonetin + NADP+
-
 ?
1.3.1.51 2'-Hydroxygenistein + NADPH 10% of the reaction relative to 2'-hydroxydaidzein Glycine max 2'-Hydroxydihydrogenistein + NADP+
-
 ?
1.3.1.51 additional information no activity with: biochanin A, daidzein, genistein, apigenin, kaempferol, quercetin Glycine max ?
-
 ?
1.3.1.51 additional information specific role in the biosynthesis of glyceollin Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.51 monomer 1 * 34700, SDS-PAGE Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.51 7
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.51 5.5 9 pH 5.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity Glycine max