Any feedback?
Literature summary extracted from
- Isolation, cloning, and sequencing of the Salmonella typhimurium ddlA gene with purification and characterization of its product, D-alanine:D-alanine ligase (ADP forming) (1988), Biochemistry, 27, 3701-3708.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.3.2.4 | subcloned behind the tac promoter in the plasmid pKK223-3, expression in Escherichia coli JM105 | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.4 | 39271 | - |
x * 39271, calculation from nucleotide sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
| 6.3.2.4 | 42000 | - |
x * 42000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.3.2.4 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.3.2.4 | overproducing strain | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.4 | 16.4 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.2.4 | ATP + D-Ala + D-Ala | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + D-Ala-D-Ala | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.3.2.4 | ? | x * 42000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 6.3.2.4 | ? | x * 39271, calculation from nucleotide sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.4 | 63 | - |
5 min, complete inactivation | Salmonella enterica subsp. enterica serovar Typhimurium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
