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Literature summary extracted from

  • Huber, C.; Skopan, H.; Feicht, R.; White, H.; Simon, H.
    Pterin cofactor, substrate specificity, and observations on the kinetics of the reversible tungsten-containing aldehyde oxidoreductase from Clostridium thermoaceticum (1995), Arch. Microbiol., 164, 110-118.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.99.6 2-Hydroxypyridine-5-carboxylate
-
 Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.99.6 0.005
-
 4-methoxybenzaldehyde terephthalaldehyde, with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.005
-
 4-methoxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.006
-
 Butanal with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.008
-
 propanal with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.009
-
 benzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.01
-
 ES-1 ferredoxin acetaldehyde, with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.012
-
 cinnamaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.02
-
 benzaldehyde 3,4-dimethoxy-5-hydroxybenzaldehyde, with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.03
-
 reduced carbamoyl methyl viologen with propanoate as cosubstrate Moorella thermoacetica
1.2.99.6 0.05
-
 Indoleacetaldehyde reduced carbamoyl methyl viologen, with benzoate as cosubstrate Moorella thermoacetica
1.2.99.6 0.1
-
 5-Phenyl-2,4-pentadienate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 0.16
-
 reduced carbamoyl methyl viologen with benzoate as cosubstrate Moorella thermoacetica
1.2.99.6 1
-
 4-Chlorobenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 1.6
-
 methanal with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 1.6
-
 methanal 4-fluorobenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 1.6
-
 methanal 4-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 1.7
-
 cinnamate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 2.4
-
 3-chlorobenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 2.9
-
 methyl viologen 3-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 3
-
 4-hydroxy-3-methoxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 3
-
 4-hydroxy-3-methoxybenzaldehyde 4-methylbenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 3.5
-
 3-Fluorobenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 4
-
 terephthalate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 5.6
-
 acetate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 6.1
-
 3-methylbenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 6.3
-
 propanoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 8
-
 4-(hydroxymethyl)benzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 10
-
 3,5-dimethoxy-4-hydroxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 12
-
 4-hydroxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 31
-
 benzoate with reduced carbamoyl methyl viologen as cosubstrate, pH 4.5 Moorella thermoacetica
1.2.99.6 53
-
 benzoate with reduced carbamoyl methyl viologen as cosubstrate, pH 5.5 Moorella thermoacetica
1.2.99.6 78
-
 3-hydroxybenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 78
-
 3-hydroxybenzoate pyridine-4-carboxylate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.2.99.6 130
-
 4-hydroxybenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
1.2.99.6 Moorella thermoacetica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.99.6 3,4-Dimethoxy-5-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
 Moorella thermoacetica 3,4-Dimethoxy-5-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 3,5-Dimethoxy-4-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
 Moorella thermoacetica 3,5-Dimethoxy-4-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-(Hydroxymethyl)benzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-(Hydroxymethyl)benzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Hydroxy-3-methoxy-benzaldehyde + carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Hydroxy-3-methoxy-benzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Hydroxybenzaldehyde + carbamoyl methyl viologen r Moorella thermoacetica 4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Methoxybenzaldehyde + benzyl viologen
-
 Moorella thermoacetica 4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 5-Phenyl-2,4-pentadienate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica ? + carbamoyl methyl viologen
-
 ?
1.2.99.6 Acetaldehyde + carbamoyl methyl viologen r Moorella thermoacetica acetate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 Benzaldehyde + carbamoyl methyl viologen r Moorella thermoacetica Benzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 Butanal + NH2CO-methyl viologen
-
 Moorella thermoacetica Butanoate + reduced NH2CO-methyl viologen
-
 ?
1.2.99.6 Cinnamaldehyde + carbamoyl methyl viologen r Moorella thermoacetica Cinnamate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 Indoleacetaldehyde + benzyl viologen
-
 Moorella thermoacetica Indoleacetate + reduced benzyl viologen
-
 ?
1.2.99.6 Methanal + carbamoyl methyl viologen
-
 Moorella thermoacetica Formate + reduced methyl viologen
-
 ?
1.2.99.6 Propanal + methyl viologen
-
 Moorella thermoacetica Propanoate + reduced methyl viologen
-
 ?
1.2.99.6 Propanal + NH2CO-methyl viologen r Moorella thermoacetica Propanoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 Pyridine 4-carboxylate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica Pyridine 4-carboxaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 terephthalaldehyde + carbamoyl methyl viologen r Moorella thermoacetica terephthalate + reduced carbamoyl methyl viologen
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.99.6 46
-
 at pH 4.5 Moorella thermoacetica
1.2.99.6 51
-
 propanoate as substrate, pH 4.8 Moorella thermoacetica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.99.6 51 71 51°C: optimum, 71°C: about 60% of maximal activity, propanoate and reduced carbamoyl methyl viologen as substrates Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.99.6 4.1
-
 4-fluorobenzoate Moorella thermoacetica
1.2.99.6 4.4 4.7 benzoate and reduced carbamoyl methyl viologen as substrate Moorella thermoacetica
1.2.99.6 4.7
-
 propanoate and reduced carbamoyl methyl viologen as substrate Moorella thermoacetica
1.2.99.6 4.7
-
 4-methoxybenzoate Moorella thermoacetica
1.2.99.6 4.7
-
 4-methoxybenzoate and reduced carbamoyl methyl viologen as substrate Moorella thermoacetica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.99.6 3.9 4.1 pH 3.9: 80% of maximal activity, pH 4.1: optimum, 4-fluorobenzoate and reduced carbamoyl methyl viologen as substrates Moorella thermoacetica
1.2.99.6 3.9 4.7 pH 3.9: 55% of maximal activity, pH 4.7: optimum, 4-methoxybenzoate and reduced carbamoyl methyl viologen as substrates Moorella thermoacetica
1.2.99.6 3.9 4.7 pH 3.9: 55% of maximal activity, pH 4.3-4.7: optimum Moorella thermoacetica
1.2.99.6 4.7 5.5 pH 4.7: optimum, pH 5.5: 20% of maximal activity, propanoate reduced carbamoyl methyl viologen as substrates Moorella thermoacetica