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Literature summary extracted from

  • Heider, J.; Ma, K.; Adams, M.W.W.
    Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1 (1995), J. Bacteriol., 177, 4757-4764.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.5 2,2'-bipyridyl competitive to methyl viologen Thermococcus sp.
1.2.7.5 acetaldehyde 50% inhibition at 5 mM Thermococcus sp.
1.2.7.5 aldehydes substrate inhibition at high concentrations, overview Thermococcus sp.
1.2.7.5 arsenite 50% inhibition at 0.5 mM Thermococcus sp.
1.2.7.5 crotonaldehyde 50% inhibition at 20 mM Thermococcus sp.
1.2.7.5 iodoacetate 50% inhibition at 0.5 mM Thermococcus sp.
1.2.7.5 isovalerylaldehyde 50% inhibition at 4 mM Thermococcus sp.
1.2.7.5 p-chloromercuribenzoate
-
 Thermococcus sp.
1.2.7.5 phenylacetaldehyde 50% inhibition at 7 mM Thermococcus sp.
1.2.7.5 propionaldehyde 50% inhibition at 0.6 mM Thermococcus sp.
1.2.7.5 Zn2+ 50% inhibition at 0.25 mM Thermococcus sp.
1.2.99.6 2,2'-bipyridyl
-
 Thermococcus sp.
1.2.99.6 acetaldehyde 5 mM, 50% inhibition Thermococcus sp.
1.2.99.6 AsO2-
-
 Thermococcus sp.
1.2.99.6 CN- relatively insensitive, 10% inhibition after 1 with 8 mM Thermococcus sp.
1.2.99.6 iodoacetate
-
 Thermococcus sp.
1.2.99.6 Isovaleraldehyde 4 mM, 50% inhibition Thermococcus sp.
1.2.99.6 PCMB
-
 Thermococcus sp.
1.2.99.6 phenylacetaldehyde 7 mM, 50% inhibition Thermococcus sp.
1.2.99.6 ZnCl2
-
 Thermococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.5 0.01
-
 oxidized ferredoxin ES-1 ferredoxin Thermococcus sp.
1.2.7.5 0.01
-
 oxidized ferredoxin pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
1.2.7.5 0.016
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.028
-
 isovalerylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.05
-
 Indoleacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.057
-
 benzaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.065
-
 Salicylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.076
-
 phenylacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.13
-
 crotonaldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.14
-
 crotonaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.15
-
 propionaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.17
-
 benzyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
1.2.7.5 0.2
-
 glyceraldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 1.42
-
 formaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 1.8
-
 acetate pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 2.9
-
 methyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
1.2.99.6 0.01
-
 ES-1 ferredoxin with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.016
-
 acetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.028
-
 Isovaleraldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.05
-
 Indoleacetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.057
-
 benzaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.065
-
 Salicylaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.076
-
 phenylacetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.13
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
1.2.99.6 0.136
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.15
-
 propanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.169
-
 benzyl viologen with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 0.196
-
 glyceraldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
1.2.99.6 1.422
-
 methanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 1.8
-
 acetate with methylviologen as cosubstrate Thermococcus sp.
1.2.99.6 2.9
-
 methyl viologen with crotonaldehyde as cosubstrate Thermococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.5 Ca2+ 0.7 gatoms per subunit Thermococcus sp.
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Thermococcus sp.
1.2.7.5 Mg2+ contains 1 Mg-atom per subunit Thermococcus sp.
1.2.7.5 Molybdenum
-
 Thermococcus sp.
1.2.7.5 Tungsten 1 tungsten per subunit Thermococcus sp.
1.2.7.5 Zn2+ 0.2 gatoms per subunit Thermococcus sp.
1.2.99.6 Ca2+ the enzyme contains 0.7 gatom of Ca per mol of enzyme Thermococcus sp.
1.2.99.6 Iron enzyme contains 3.6 gatom Fe per mol of enzyme Thermococcus sp.
1.2.99.6 Iron contains a single [4Fe-4S]+cluster with an S = 3/2 ground state Thermococcus sp.
1.2.99.6 Mg2+ enzyme contains 1.1 gatom of Mg per mol of enzyme Thermococcus sp.
1.2.99.6 Tungsten enzyme contains 0.9 gatom of tungsten per mol of enzyme Thermococcus sp.
1.2.99.6 Zn2+ the enzyme contains 0.2 gatom of Zn per mol of enzyme Thermococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.5 67000
-
 2 * 67000, SDS-PAGE Thermococcus sp.
1.2.7.5 105000
-
 gel filtration Thermococcus sp.
1.2.99.6 67000
-
 2 * 67000, SDS-PAGE Thermococcus sp.
1.2.99.6 105000
-
 gel filtration Thermococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Thermococcus sp. enzyme oxidizes aldehydes generated during amino acid catabolism an acid + H+ + reduced ferredoxin
-
 ?
1.2.99.6 additional information Thermococcus sp. the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation ?
-
 ?
1.2.99.6 additional information Thermococcus sp. ES-1 the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation ?
-
 ?
1.2.99.6 additional information Thermococcus sp. ES1 the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.5 Thermococcus sp.
-
 hyperthermophilic archaeon
-
1.2.7.5 Thermococcus sp. ES1
-
 hyperthermophilic archaeon
-
1.2.99.6 Thermococcus sp.
-
-
-
1.2.99.6 Thermococcus sp. ES-1
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.5 very sensitive to O2, after 1 h at 60°C 3% activity remains, after 1 h at 4°C 39% activity remains Thermococcus sp.
1.2.99.6 extremely sensitive to inactivation by O2. The extent of inactivation is greatly dependent on the temperature of incubation Thermococcus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.5 about 120fold, to homogeneity Thermococcus sp.
1.2.99.6
-
 Thermococcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.99.6 culture condition:ethanol-grown cell
-
 Thermococcus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.5 238
-
 purified enzyme Thermococcus sp.
1.2.99.6 221
-
-
 Thermococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. acetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 acetaldehyde + H2O + oxidized ferredoxin best substrate Thermococcus sp. acetate + H+ + reduced ferredoxin
-
 ir
1.2.7.5 acetate + H+ + reduced methyl viologen very low activity Thermococcus sp. acetaldehyde + H2O + oxidized methyl viologen
-
 ir
1.2.7.5 acetate + H+ + reduced methyl viologen below pH 6.0 Thermococcus sp. acetaldehyde + H2O + oxidized methyl viologen
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin enzyme oxidizes aldehydes generated during amino acid catabolism Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 benzaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. benzoic acid + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. crotonate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 crotonaldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. crotonate + H+ + reduced ferredoxin
-
 r
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. formate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 glyceraldehyde + H2O + oxidized methyl viologen very poor substrate Thermococcus sp. glycerate + H+ + reduced methyl viologen
-
 ?
1.2.7.5 indoleacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. indoleacetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 isovalerylaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. isovalerate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 additional information acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate Thermococcus sp. ?
-
 ?
1.2.7.5 phenylacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. phenylacetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 propionaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. propionate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 salicylaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. salicylic acid + H+ + reduced benzyl viologen
-
 ir
1.2.99.6 Acetaldehyde + benzyl viologen r Thermococcus sp. acetate + reduced benzyl viologen
-
 ?
1.2.99.6 Acetaldehyde + benzyl viologen r Thermococcus sp. ES-1 acetate + reduced benzyl viologen
-
 ?
1.2.99.6 Acetaldehyde + benzyl viologen r Thermococcus sp. ES1 acetate + reduced benzyl viologen
-
 ?
1.2.99.6 Benzaldehyde + benzyl viologen
-
 Thermococcus sp. Benzoate + reduced benzyl viologen
-
 ?
1.2.99.6 Benzaldehyde + benzyl viologen
-
 Thermococcus sp. ES-1 Benzoate + reduced benzyl viologen
-
 ?
1.2.99.6 Benzaldehyde + benzyl viologen
-
 Thermococcus sp. ES1 Benzoate + reduced benzyl viologen
-
 ?
1.2.99.6 Crotonaldehyde + benzyl viologen at less than 1% of the activity with benzyl viologen Thermococcus sp. E-2-Butenoate + reduced benzyl viologen
-
 ?
1.2.99.6 Crotonaldehyde + ES-1 ferredoxin
-
 Thermococcus sp. Crotonate + reduced ES-1 ferredoxin
-
 ?
1.2.99.6 Crotonaldehyde + methyl viologen
-
 Thermococcus sp. Crotonate + reduced methyl viologen
-
 ?
1.2.99.6 Glyceraldehyde + benzyl viologen
-
 Thermococcus sp. Glycerate + reduced benzyl viologen
-
 ?
1.2.99.6 Indoleacetaldehyde + benzyl viologen
-
 Thermococcus sp. Indoleacetate + reduced benzyl viologen
-
 ?
1.2.99.6 Isovaleraldehyde + benzyl viologen
-
 Thermococcus sp. Isovalerate + reduced benzyl viologen
-
 ?
1.2.99.6 Isovaleraldehyde + benzyl viologen
-
 Thermococcus sp. ES-1 Isovalerate + reduced benzyl viologen
-
 ?
1.2.99.6 Isovaleraldehyde + benzyl viologen
-
 Thermococcus sp. ES1 Isovalerate + reduced benzyl viologen
-
 ?
1.2.99.6 Methanal + benzyl viologen
-
 Thermococcus sp. Formate + reduced benzyl viologen
-
 ?
1.2.99.6 additional information the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation Thermococcus sp. ?
-
 ?
1.2.99.6 additional information the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation Thermococcus sp. ES-1 ?
-
 ?
1.2.99.6 additional information the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation Thermococcus sp. ES1 ?
-
 ?
1.2.99.6 Phenylacetaldehyde + benzyl viologen
-
 Thermococcus sp. Phenylacetate + reduced benzyl viologen
-
 ?
1.2.99.6 Phenylacetaldehyde + benzyl viologen
-
 Thermococcus sp. ES-1 Phenylacetate + reduced benzyl viologen
-
 ?
1.2.99.6 Phenylacetaldehyde + benzyl viologen
-
 Thermococcus sp. ES1 Phenylacetate + reduced benzyl viologen
-
 ?
1.2.99.6 Propanal + benzyl viologen
-
 Thermococcus sp. Propanoate + reduced benzyl viologen
-
 ?
1.2.99.6 Salicylaldehyde + benzyl viologen
-
 Thermococcus sp. Salicylate + reduced benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.5 dimer 2 * 67000, SDS-PAGE Thermococcus sp.
1.2.99.6 dimer 2 * 67000, SDS-PAGE Thermococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.5 85
-
 assay at Thermococcus sp.
1.2.99.6 85
-
-
 Thermococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.5 additional information
-
 the enzyme is thermostable Thermococcus sp.
1.2.7.5 23
-
 pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min Thermococcus sp.
1.2.99.6 70
-
 enzyme concentration 2.0 mg/ml, 50% loss of activity after 5 min Thermococcus sp.
1.2.99.6 85
-
 enzyme concentration 2.0 mg/ml, 50% loss of activity after 1 min Thermococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.5 1.6
-
 acetate pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 2.8
-
 glyceraldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 13
-
 Salicylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 22
-
 crotonaldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 55
-
 Indoleacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 62
-
 methyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
1.2.7.5 250
-
 benzyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
1.2.7.5 269
-
 crotonaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 270
-
 oxidized ferredoxin ES-1 ferredoxin, pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
1.2.7.5 272
-
 isovalerylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 343
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 720
-
 benzaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 950
-
 formaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 960
-
 phenylacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 1100
-
 propionaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.99.6 1.6
-
 acetaldehyde with methyl viologen as cosubstrate Thermococcus sp.
1.2.99.6 10.5
-
 glyceraldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
1.2.99.6 13
-
 Salicylaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 22
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 45°C Thermococcus sp.
1.2.99.6 55
-
 indolecetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 62
-
 methyl viologen with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 250
-
 benzyl viologen with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 269
-
 crotonaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 270
-
 ES-1 ferredoxin with crotonaldehyde as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 272
-
 Isovaleraldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 343
-
 acetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 720
-
 benzaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 950
-
 methanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 960
-
 phenylacetaldehyde with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.
1.2.99.6 1100
-
 propanal with benzyl viologen as cosubstrate, at 85°C Thermococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.5 8.4
-
 assay at Thermococcus sp.
1.2.99.6 8
-
 around, at 80 C, with benzyl viologen and crotonaldehyde as substrate Thermococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.5 6 10 oxidation of acetaldehyde with benzyl viologen as electron acceptor Thermococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.5 Ferredoxin
-
 Thermococcus sp.
1.2.7.5 form A cofactor in presence of iodine Thermococcus sp.
1.2.7.5 form B cofactor in absence of iodine Thermococcus sp.
1.2.7.5 molybdopterin
-
 Thermococcus sp.
1.2.99.6 molybdopterin enzyme contains molybdopterin Thermococcus sp.