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Literature summary extracted from

  • Gushima, H.; Miya, T.; Murata, K.; Kimura, A.
    Purification and characterization of glutathione synthetase from Escherichia coli B (1983), J. Appl. Biochem., 5, 210-218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 Escherichia coli C600 cells transformed by a recombinant plasmid for the glutathione synthetase gene of Escherichia coli B Escherichia coli

General Stability

EC Number General Stability Organism
6.3.2.3 relatively stable at all stages of purification Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 adenosine
-
 Escherichia coli
6.3.2.3 ADP
-
 Escherichia coli
6.3.2.3 AMP
-
 Escherichia coli
6.3.2.3 GSSG no inhibition by reduced glutathione Escherichia coli
6.3.2.3 Hg2+
-
 Escherichia coli
6.3.2.3 PCMB
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 1.8
-
 ATP
-
 Escherichia coli
6.3.2.3 2
-
 Gly
-
 Escherichia coli
6.3.2.3 2.6
-
 gamma-Glu-Cys
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.3 Co2+ divalent metal ion required, Mg2+ is most effective, Co2+ can replace Mg2+ Escherichia coli
6.3.2.3 Mg2+ divalent metal ion required, Mg2+ is most effective Escherichia coli
6.3.2.3 Mn2+ can replace Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.3 38000
-
 x * 38000, SDS-PAGE Escherichia coli
6.3.2.3 152000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Escherichia coli
-
 B
-
6.3.2.3 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 10.83
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.3.2.3 -20°C, stable for weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + glutathione
-
 ?
6.3.2.3 UTP + gamma-Glu-L-Cys + Gly 10% of the activity relative to ATP Escherichia coli UDP + phosphate + glutathione
-
 ?
6.3.2.3 UTP + gamma-Glu-L-Cys + Gly 10% of the activity relative to ATP Escherichia coli B / ATCC 11303 UDP + phosphate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.3 ? x * 38000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.3 37
-
 stable below Escherichia coli
6.3.2.3 60
-
 15 min, 50% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.3 5 7.5 stable Escherichia coli