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Literature summary extracted from

  • Kato, H.; Chihara, M.; Nishioka, T.; Murata, K.; Kimura, A.; Oda, J.
    Homology of Escherichia coli B glutathione synthetase with dihydrofolate reductase in amino acid sequence and substrate binding site (1987), J. Biochem., 101, 207-215.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 the gene for glutathione synthetase of Escherichia coli B is cloned onto vector plasmid pBR325. Escherichia coli C600 cells transformed by a recombinant plasmid pBR325 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 7,8-dihydrofolate
-
 Escherichia coli
6.3.2.3 ATP substrate inhibition above 15 mM Escherichia coli
6.3.2.3 methotrexate
-
 Escherichia coli
6.3.2.3 NADPH weak Escherichia coli
6.3.2.3 trimethoprim
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 1.85
-
 ATP
-
 Escherichia coli
6.3.2.3 3.33
-
 gamma-Glu-Cys
-
 Escherichia coli
6.3.2.3 3.53
-
 Gly
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Escherichia coli
-
 B
-
6.3.2.3 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 42.83
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + glutathione
-
 ?