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Literature summary extracted from

  • Zwickl, P.; Fabry, S.; Bogedain, C.; Haas, A.; Hensel, R.
    Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli (1990), J. Bacteriol., 172, 4329-4338.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.13
-
 Pyrococcus woesei
1.2.1.59 expression at high levels in Escherichia coli, the primary structure exhibits a strikingly high proportion of aromatic amino acids Pyrococcus woesei

General Stability

EC Number General Stability Organism
1.2.1.59 several salts stabilize against thermal inactivation, K+-citrate is most effective Pyrococcus woesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.59 additional information insensitive to pentalenolactone Pyrococcus woesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 0.01
-
 NADP+
-
 Pyrococcus woesei
1.2.1.13 1
-
 NAD+
-
 Pyrococcus woesei
1.2.1.13 40
-
 phosphate
-
 Pyrococcus woesei
1.2.1.13 120
-
 arsenate
-
 Pyrococcus woesei
1.2.1.59 0.01
-
 NADP+
-
 Pyrococcus woesei
1.2.1.59 1
-
 NAD+
-
 Pyrococcus woesei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.59 phosphate dependent on Pyrococcus woesei
1.2.1.59 phosphate Km: 0.04 mM Pyrococcus woesei

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Pyrococcus woesei
-
-
-
1.2.1.59 Pyrococcus woesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.13
-
 Pyrococcus woesei
1.2.1.59
-
 Pyrococcus woesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.13 additional information
-
-
 Pyrococcus woesei
1.2.1.59 additional information
-
-
 Pyrococcus woesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
 Pyrococcus woesei 3-phospho-D-glyceroyl arsenate + NADPH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrococcus woesei 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Pyrococcus woesei 3-phospho-D-glyceroyl phosphate + NADPH
-
 ?
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrococcus woesei 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
1.2.1.59 D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Pyrococcus woesei 1,3-diphosphoglycerate + NAD(P)H
-
 ?
1.2.1.59 D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Pyrococcus woesei 1,3-diphosphoglycerate + NADPH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.13 additional information
-
 sodium chloride, potassium chloride, sodium sulfate, potassium sulfate, sodium phosphate, potassium phosphate, sodium citrate and potassium citrate stabilize against thermal inactivation at 104°C. K+ is more effective than Na+ with respect to anion Pyrococcus woesei
1.2.1.13 97
-
 half-life: 65 min Pyrococcus woesei
1.2.1.13 100
-
 half-life: 44 min Pyrococcus woesei
1.2.1.13 104
-
 half-life: 9 min without stabilizer, stable for 30 min in presence of 250 mM potassium citrate Pyrococcus woesei
1.2.1.59 100
-
 half-life: 44 min Pyrococcus woesei

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.13 NAD+ cofactor Pyrococcus woesei
1.2.1.13 NADP+ cofactor Pyrococcus woesei