Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, J.; Hegeman, C.E.; Hanlon, R.W.; Lacy, G.H.; Denbow, D.M.; Grabau, E.A.
    Secretion of active recombinant phytase from soybean cell-suspension cultures (1997), Plant Physiol., 114, 1103-1111.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Glycine max, inserted into soybean transformation plasmid under control of constitutive and seed-specific promoters Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 4°C, stable for 2 months Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 58
-
 at pH 5.5 Aspergillus niger
3.1.3.8 63
-
 at pH 5.0 Aspergillus niger
3.1.3.8 66
-
 at pH 4.5 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 63
-
 10 min, 10% loss of the activity of the recombinant phytase, 25% loss of the activity of the fungal enzyme Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 3
-
 at 58°C, and a second optimum at pH 5.5 Aspergillus niger
3.1.3.8 5.5
-
 at 58°C, and a second optimum at pH 3.0 Aspergillus niger