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Literature summary extracted from

  • Chen, L.; Roberts, M.F.
    Characterization of a tetrameric inositol monophosphatase from the hyperthermophilic bacterium Thermotoga maritima (1999), Appl. Environ. Microbiol., 65, 4559-4567.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 overexpression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+ 50% inhibitory at 100 mM Thermotoga maritima
3.1.3.25 Mg2+ slight inhibition at 100 mM Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.145
-
 DL-myo-inositol 1-phosphate
-
 Thermotoga maritima
3.1.3.25 0.148
-
 D-inositol 1-phosphate
-
 Thermotoga maritima
3.1.3.25 0.35
-
 inositol-2-phosphate
-
 Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Co2+
-
 Thermotoga maritima
3.1.3.25 Mg2+
-
 Thermotoga maritima
3.1.3.25 Mn2+
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 11400
-
 sucrose density sedimentation Thermotoga maritima
3.1.3.25 28000
-
 4 * 28000, SDS-PAGE Thermotoga maritima
3.1.3.25 116000
-
 chromatography on bio-gel as well as gel electrophoresis Thermotoga maritima
3.1.3.25 119000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Thermotoga maritima
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.25 442
-
-
 Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
3.1.3.25 4°C, no loss of activity for 1 month Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O
-
 Thermotoga maritima adenosine + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O
-
 Thermotoga maritima 4-nitrophenol + phosphate
-
 ?
3.1.3.25 D-inositol 1-phosphate + H2O
-
 Thermotoga maritima D-inositol + phosphate
-
 ?
3.1.3.25 DL-myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima DL-myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 2-phosphate + H2O
-
 Thermotoga maritima myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 tetramer 4 * 28000, SDS-PAGE Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 85
-
 for 30 min no loss of activity Thermotoga maritima
3.1.3.25 100
-
 for 10 min 20-25%, for 30 min 70% loss of activity Thermotoga maritima