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Literature summary extracted from

  • Chen, L.; Roberts, M.F.
    Cloning and expression of the inositol monophosphatase gene from Methanococcus jannaschii and characterization of the enzyme (1998), Appl. Environ. Microbiol., 64, 2609-2615.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expression of the MJ109 gene in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Ba2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Ca2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Co2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Cu2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Li+ 250 mM inhibits about 65%, 1000 mM about 90% Methanocaldococcus jannaschii
3.1.3.25 Ni2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Zn2+
-
 Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Li+ at 100 mM the activity is slightly enhanced Methanocaldococcus jannaschii
3.1.3.25 Mg2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Mn2+
-
 Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 28000
-
 2 * 28000, SDS-PAGE Methanocaldococcus jannaschii
3.1.3.25 55000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Methanocaldococcus jannaschii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.25 9.3
-
-
 Methanocaldococcus jannaschii

Storage Stability

EC Number Storage Stability Organism
3.1.3.25 4°C, no loss of activity for 1 month Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O
-
 Methanocaldococcus jannaschii adenosine + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O high rate of hydrolysis Methanocaldococcus jannaschii 4-nitrophenol + phosphate
-
 ?
3.1.3.25 glucose 1-phosphate + H2O high rate of hydrolysis Methanocaldococcus jannaschii ?
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer 2 * 28000, SDS-PAGE Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 85
-
 for 30 min 95% of activity remains Methanocaldococcus jannaschii